Difference between revisions of "Part:BBa K5049001"
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The sequences of DNA elements for the GTH1 promoter and GCW61 anchor protein were from Pichia pastoris. To ensure flexibility in connecting to target fusion proteins, a GS linker ((GGSG)3) was added at the N-terminus of GCW61.  
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The sequence of DNA elements for GCW61 anchor protein were from Pichia pastoris. To ensure flexibility in connecting to target fusion proteins, a GS linker ((GGSG)3) was added at the N-terminus of GCW61.The GS-GCW61 DNA fragment was synthesized by IDT and cloned into pSB1C3 as a basic part. 
 
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Revision as of 03:38, 10 September 2024


GS-GCW61 (GCW61 anchor protein with a GS linker)

The sequence of DNA elements for GCW61 anchor protein were from Pichia pastoris. To ensure flexibility in connecting to target fusion proteins, a GS linker ((GGSG)3) was added at the N-terminus of GCW61.The GS-GCW61 DNA fragment was synthesized by IDT and cloned into pSB1C3 as a basic part.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 187
  • 1000
    COMPATIBLE WITH RFC[1000]