Difference between revisions of "Part:BBa K174014"

 
 
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<partinfo>BBa_K174014 short</partinfo>
 
<partinfo>BBa_K174014 short</partinfo>
  
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Both CwlJ and SleB are germination specific cortex hydrolases. Although SleB has a major effect for the germination, cwlJ's effect is minor. However bacillus subtilis with double mutation without cwlJ and sleB were not able germinate again (2).
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Both CwlJ and SleB are germination specific cortex hydrolases [1, 2]. Although SleB has a major effect for the germination, cwlJ's effect is minor. However ''Bacillus subtilis'' with double mutation without cwlJ and sleB were not able germinate again [2].
  
 
To control the germination rate tightly, cwlJ and sleB genes are combined to make this device.
 
To control the germination rate tightly, cwlJ and sleB genes are combined to make this device.
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<partinfo>BBa_K174014 parameters</partinfo>
 
<partinfo>BBa_K174014 parameters</partinfo>
 
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===References===
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#Moriyama, R., A. Hattori, S. Miyata, S. Kudoh, and S. Makino. 1996. A gene (sleB) encoding a spore cortex-lytic enzyme from Bacillus subtilis and response of the enzyme to l-alanine-mediated germination. J. Bacteriol. 178:6059-6063
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#Ishikawa, S., K. Yamane, and J. Sekiguchi. 1998. Regulation and characterization of a newly deduced cell wall hydrolase gene (cwlJ) which affects germination of Bacillus subtilis spores. J. Bacteriol. 180:1375-138

Latest revision as of 10:47, 21 October 2009

cwlJ + sleB, B. subtilis germination genes

Both CwlJ and SleB are germination specific cortex hydrolases [1, 2]. Although SleB has a major effect for the germination, cwlJ's effect is minor. However Bacillus subtilis with double mutation without cwlJ and sleB were not able germinate again [2].

To control the germination rate tightly, cwlJ and sleB genes are combined to make this device.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Moriyama, R., A. Hattori, S. Miyata, S. Kudoh, and S. Makino. 1996. A gene (sleB) encoding a spore cortex-lytic enzyme from Bacillus subtilis and response of the enzyme to l-alanine-mediated germination. J. Bacteriol. 178:6059-6063
  2. Ishikawa, S., K. Yamane, and J. Sekiguchi. 1998. Regulation and characterization of a newly deduced cell wall hydrolase gene (cwlJ) which affects germination of Bacillus subtilis spores. J. Bacteriol. 180:1375-138