Difference between revisions of "Part:BBa K174011:Design"
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===References===
 
===References===
 +
#Veening, J.-W., W. K. Smits, et al. (2008). "Bistability, Epigenetics, and Bet-Hedging in Bacteria." Annual Review of Microbiology 62(1): 193-210.
 +
#Fujita, M. and R. Losick (2005). "Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A." Genes & Development 19(18): 2236-2244.

Revision as of 10:44, 21 October 2009

IPTG inducable kinA Bacillus sporulation trigger


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In B. subtilis, out of five kinases only KinA, KinB and KinC kinases are effective to trigger the sporulation. KinD and KinE do not phosphorylate Spo0F, hence do not effect the sporulation. We chose KinA for our device.

As our whole system grew, it was not possible to test everything. Rather than LacI which can be derepressed by IPTG, we had another transcription factor that represses the device's promoter. Instead, we designed our promoter with flanking sites to be induced by IPTG and unit test it. The idea was to construct everything and plug them all together after we test each component.


Source

Synthesised.

References

  1. Veening, J.-W., W. K. Smits, et al. (2008). "Bistability, Epigenetics, and Bet-Hedging in Bacteria." Annual Review of Microbiology 62(1): 193-210.
  2. Fujita, M. and R. Losick (2005). "Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A." Genes & Development 19(18): 2236-2244.