Difference between revisions of "Part:BBa K180001"

 
 
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<partinfo>BBa_K180001 short</partinfo>
 
<partinfo>BBa_K180001 short</partinfo>
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Based on the part E0040, but with an LVA degradation tail attached.
 
Based on the part E0040, but with an LVA degradation tail attached.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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LVA tag addition to end of green fluorescent protein sequence to increase the rate of degradation, which leads to GFP expression for a shorter time than the original part, E0040. This may be of use if a user is trying to monitor GFP expression under changing environmental conditions, when it would be undesirable to have GFP continually expressed for long periods of time.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K180001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K180001 SequenceAndFeatures</partinfo>

Latest revision as of 10:41, 21 October 2009

Green fluorescent protein (+LVA)

Based on the part E0040, but with an LVA degradation tail attached.


Usage and Biology

LVA tag addition to end of green fluorescent protein sequence to increase the rate of degradation, which leads to GFP expression for a shorter time than the original part, E0040. This may be of use if a user is trying to monitor GFP expression under changing environmental conditions, when it would be undesirable to have GFP continually expressed for long periods of time.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644