Difference between revisions of "Part:BBa K5082009"

Revision as of 10:44, 7 September 2024

Usage and Biology

Plasmids are double stranded circular DNA molecules, which carry non-essential genes, found in bacterial cells [1]. Plasmids are multifunction, easy to edit, and can be amplified quickly by bacterial cellular machinery [2]. These advantages have made plasmids a popular tool in genetic research.

pHAGE is a mammalian expression vector [3]. The pHAGE plasmid can be amplified by DH5α bacterial cells. Meanwhile, it carries an ampicillin resistance gene for selection. The plasmid map for pCMV-EGFP is shown in Figure 1.

         
            Figure 1. pHAGE plasmid map.

Design

The above characteristics have made pHAGE an ideal plasmid for our experiment. In our experiment, we inserted a G3BP1 gene into the plasmid. Upon amplification, we transfected the plasmid into stomach cells at different concentrations to measure the sensitivity of the sensor we designed. The results were as expected, proving the effectiveness of this plasmid backbone.

Reference

[1] Carattoli, Alessandra. “Plasmids in Gram Negatives: Molecular Typing of Resistance Plasmids.” International Journal of Medical Microbiology, vol. 301, no. 8, Dec. 2011, pp. 654–658

[2] Patron, N.J. Synthetic Biology and Gene Cloning. Elsevier EBooks, Elsevier BV, 1 Jan. 2017, pp. 112–117

[3] “Addgene: PTS106 PHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP.” Addgene.org, 2024, www.addgene.org/199359/. Accessed 15 Aug. 2024.

Sequence and Features