Difference between revisions of "Part:BBa K5398002"
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This part is a part of the circular mRNA (cmRNA) (3´ side).
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To generate the squid ring proteins with various long tandem repeats (TRn), we utilized the td intron, an intron of the td gene from T4 phage belonging to group IE, which circularizes the exon to form a back-splice junction (BSJ) in a reaction catalyzed by guanosine. To ensure that the ribosomes do not translate the ORF of TRn from unprocessed linear mRNA, the ribosome binding sequence (RBS) and translation initiation codon ATG were placed downstream of the TRn coding sequence. Consequently, the regulatory sequences were located upstream of the coding sequence only after circularization of the mRNA. To purify the resulting TRn polypeptides, a His tag was incorporated into the ORF. If the mRNA is circularized, the ribosome could circle the cmRNA, producing a long repeating polypeptide.
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__NOTOC__
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<partinfo>BBa_K5398002 short</partinfo>
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This part codes for the biosynthetic proteins TRn5, composed of squid ring teeth proteins with five tandem repeats. TRn5 can connect with other squid ring proteins through their common β-sheet. Given the positive correlation between number of repeat units and magnitude of cohesive force, we used TRn5 as special materials to realize self-healing.  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5398002 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K5398002 parameters</partinfo>
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Revision as of 03:35, 7 September 2024


The 5' intron of td gene from T4 phage

This part codes for the biosynthetic proteins TRn5, composed of squid ring teeth proteins with five tandem repeats. TRn5 can connect with other squid ring proteins through their common β-sheet. Given the positive correlation between number of repeat units and magnitude of cohesive force, we used TRn5 as special materials to realize self-healing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 35
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]