Difference between revisions of "Part:BBa K176014"

Latest revision as of 10:25, 21 October 2009

pCon 0.15(K176008)->RBS+GFP+T

pCon 0.15(K176008)+I13504

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 705

MEASUREMENT

We measured five constitutive promoter family member ( J23100, J23101, J23103, J23107, J23109, J23115, )strength by ligated each with a 
GFP gene ( I13504) , and then moniter the OD600 and GFP expression.
K176011: J23100 + GFP ( I13504 )
K176012: J23101 + GFP ( I13504)
K176013: K176009( J23107) + GFP ( I13504)
K176014: K176008( J23115) + GFP ( I13504)
K176015: J23109 + GFP ( I13504)
K176016: J23103 + GFP ( I13504)

Model

To see the details of the model, click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here] please.

data

K176008+GFP

COLONY NUMBER	T(min)	OD600	FLU(arbitrary)	ST.FLU(ustc_st.1)	FLU/OD600(arbitrary)	ST.FLU/OD600(ustc_st.1)
Ⅰ	0	0.344	6.204666667	24.80180144	18.03682171	72.09826
Ⅰ	30	0.451	9.088666667	36.32996229	20.15225425	80.55424012
Ⅰ	60	0.555	11.31433333	45.22657926	20.38618619	81.489332
Ⅰ	90	0.65	13.382	53.49162569	20.58769231	82.29480876
Ⅰ	120	0.685	15.55033333	62.15906517	22.70121655	90.74316083
Ⅰ	150	0.888	20.59866667	82.33867637	23.1966967	92.72373465
Ⅰ	180	1.281	29.948	119.7105968	23.37861046	93.45089523
Ⅱ	0	0.358	6.817666667	27.25213521	19.04376164	76.12328272
Ⅱ	30	0.456	9.824	39.26929688	21.54385965	86.11687912
Ⅱ	60	0.55	11.94633333	47.75286139	21.72060606	86.82338434
Ⅱ	90	0.647	15.013	60.01119239	23.20401855	92.75300215
Ⅱ	120	0.679	16.711	66.79857697	24.61119293	98.37787477
Ⅱ	150	0.866	22.18966667	88.69835179	25.62317167	102.423039
Ⅱ	180	1.247	31.647	126.5019787	25.37850842	101.445051

For the details of the definition of ST.FLU and the unit ustc_st1, please click here.

plot

protocol

1. Streak a plate of the strain which contain one of the parts listed in pSB1A3 .

2. Inoculate two 3ml cultures of supplemented M9 Medium and antibiotic( Ampicillin 0.1mg/ml) with single colony from the plate.

3. Cultures were grown in test tubes（BIO BASIC INC.12ml Polypropylene Round-bottom Culture Tubes With Graduations And Dual Cap Cat.No:TD444） for 16hrs at 37℃ with shaking at 200rpm.

4. Cultures were diluted 1:100 into 3ml fresh medium and grown for 3hrs.

5. Measure the fluorescence(SHIMDZU SPECTROFLUOROPHOTOMETER RF-5301PC, 250ul quartz cell path length 10mm,501 nm excitation,514 nm emission,1.5nm slit width) and absorbance (HITACHI UV-VIS spectrophotometer U-2810 ,200ul quartz cell,path length 10mm,600nm,1.5 nm slit width) every 30 minutes in the next 4hrs.

@@ Line 19: / Line 19: @@
   K176015: J23109 + GFP ( I13504)
   K176016: J23103 + GFP ( I13504)
+==Model==
+To see the details of the model, click [http://2009.igem.org/Team:USTC/Modeling/Model-1 here] please.
 ===data===
 K176008+GFP