Difference between revisions of "Part:BBa K283005"

 
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<partinfo>BBa_K283005 short</partinfo>
 
<partinfo>BBa_K283005 short</partinfo>
  
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This part consists of a fusion protein Lpp-rbs-protein under the pLac promoter. The Lpp is a signal peptide. The ompA domain of the fusion directs the protein to the surface membrane. The pLac promoter can be repressed by LacI protein. This part lacks a terminator.  
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This part is an improved version of BBa_J36846. BBa_J36846 consists of a signal peptide of lipoprotein (lpp), five transmembrane domains of outer membrane protein A (ompA), and streptavidin, wild-type with His6 tag. It's designed to express streptavidin on the cell surface. To test if the protein successfully expressed on the outer membrane of bacteria, you might need to perform immunostainning analysis or cell fractionation and western blot.  
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It's a time-consuming work to perform above experiments. To simplify the the procedure and directly observe the expressed protein, we inserted a eGFP-coding sequence lacking a stop codon immediately upstream of streptavidin region. The resulting BBa_K283005 has been demonstrated work as expected (visit our wiki for further information).  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:52, 21 October 2009

lpp_ompA_eGFP_streptavidin


This part is an improved version of BBa_J36846. BBa_J36846 consists of a signal peptide of lipoprotein (lpp), five transmembrane domains of outer membrane protein A (ompA), and streptavidin, wild-type with His6 tag. It's designed to express streptavidin on the cell surface. To test if the protein successfully expressed on the outer membrane of bacteria, you might need to perform immunostainning analysis or cell fractionation and western blot.

It's a time-consuming work to perform above experiments. To simplify the the procedure and directly observe the expressed protein, we inserted a eGFP-coding sequence lacking a stop codon immediately upstream of streptavidin region. The resulting BBa_K283005 has been demonstrated work as expected (visit our wiki for further information).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1234
    Illegal AgeI site found at 1285
  • 1000
    COMPATIBLE WITH RFC[1000]