Difference between revisions of "Part:BBa K283000"

Revision as of 08:52, 21 October 2009

pTet-rbs-lpp_ompA_eGFP_streptavidin-double Terminators

Streptavidin protein binds strongly to the biotin molecule, and is widely used to bind biotinylated materials. This device was designed for polar expression of streptavidin on the outer membrane of bacterial cells. To facilitate the observation, the fusion protein of Lpp signal peptide, OmpA aa46-66, and streptavidin wild-type (BBa_J36848) was fused with GFP-coding sequence (BBa_e0040). The expression will display streptavidin-GFP fusion protein on the cell surface. It has been demonstrated work as expected in our system (see experience or visit our wiki).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1272
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1314
Illegal AgeI site found at 1365
1000
COMPATIBLE WITH RFC[1000]

Revision as of 07:36, 21 October 2009 (view source) HKUHKBU (Talk \| contribs) ← Older edit		Revision as of 08:52, 21 October 2009 (view source) HKUHKBU (Talk \| contribs) Newer edit →
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−	Streptavidin protein binds strongly to the biotin molecule, and is widely used to bind biotinylated materials. This device was designed for polar expression of streptavidin on the outer membrane of bacterial cells. To facilitate the observation, the fusion protein of Lpp signal peptide, OmpA aa46-66, and streptavidin wild-type (BBa_J36848) was fused with GFP-coding sequence (BBa_e0040). The expression will display streptavidin-GFP fusion protein on the cell surface. It has been demonstrated work in our system (see experience or visit our wiki).	+	Streptavidin protein binds strongly to the biotin molecule, and is widely used to bind biotinylated materials. This device was designed for polar expression of streptavidin on the outer membrane of bacterial cells. To facilitate the observation, the fusion protein of Lpp signal peptide, OmpA aa46-66, and streptavidin wild-type (BBa_J36848) was fused with GFP-coding sequence (BBa_e0040). The expression will display streptavidin-GFP fusion protein on the cell surface. It has been demonstrated work as expected in our system (see experience or visit our wiki).