Difference between revisions of "Part:BBa K218001:Design"

 
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<partinfo>BBa_K218001 short</partinfo>
 
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GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2
 
GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2
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Jeremy A. Freeman and Bonnie L. Bassler. Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. 1999. Journal of Bacteriology. Feb 1999, p. 899-906.
 
  
 
===References===
 
===References===

Revision as of 06:03, 21 October 2009

LuxOU from Vibrio harveyi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 854
    Illegal SapI.rc site found at 816


Design Notes

This part was submitted to the Registry in 2008 (K131016); however, this part was cloned again and sequenced and is being re-submitted.

LuxO and LuxU are found within the operon, therefore we have decided to keep these genes together. The internal RBS was also kept.


Source

GenBank Accession# L26221 LuxO Protein ID# AAD12736.1 LuxU Protein ID# AAD12737.2

References