Difference between revisions of "Part:BBa K5088000"

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==The Dandelion Toolbox==
 
==The Dandelion Toolbox==
 
===Overview===
 
===Overview===
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      <table id="toolbox">
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        <tr>
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</style>
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            <th>Part Identifier</th>
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            <th>Part Type</th>
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</head>
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            <th>Nickname</th>
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            <th>Part Description</th>
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<body>
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        </tr>
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<table id="toolbox">
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        <tr>
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  <tr>
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            <td><b>BBa_K5088000</b></td>
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    <th>Part Identifier</th>
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            <td>Promoter + 5'UTR</td>
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    <th>Part Type</th>
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            <td>P_PTI1</td>
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    <th>Nickname</th>
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            <td>Protein tyrosine kinase - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
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    <th>Part Description</th>
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        </tr>
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  </tr>
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        <tr>
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  <tr>
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            <td><a href='https://parts.igem.org/Part:BBa_K5088001'>BBa_K5088001</a></td>
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    <td><b>BBa_K5088000</b></td>
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            <td>Promoter + 5'UTR</td>
−
    <td>Promoter + 5’UTR</td>
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            <td>P_RPL28</td>
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    <td>P_PTI</td>
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            <td>Large subunit ribosomal protein L28e - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
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    <td>Protein tyrosine kinase - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
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        </tr>
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  </tr>
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        <tr>
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  <tr>
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            <td><a href='https://parts.igem.org/Part:BBa_K5088002'>BBa_K5088002</a></td>
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    <td><a href='https://parts.igem.org/Part:BBa_K5088001'>BBa_K5088001</a></td>
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            <td>Promoter + 5'UTR</td>
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    <td>Promoter + 5’UTR</td>
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            <td>P_GSK3B</td>
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    <td>P_RPL28</td>
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            <td>Glycogen synthase kinase 3 - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
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    <td>Large subunit ribosomal protein L28e - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
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        </tr>
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  </tr>
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        <tr>
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  <tr>
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            <td><a href='https://parts.igem.org/Part:BBa_K5088003'>BBa_K5088003</a></td>
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    <td><a href='https://parts.igem.org/Part:BBa_K5088002'>BBa_K5088002</a></td>
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            <td>Promoter + 5'UTR</td>
−
    <td>Promoter + 5’UTR</td>
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            <td>P_MGRN1</td>
−
    <td>P_GSK3B</td>
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            <td>E3 ubiquitin-protein ligase - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
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    <td>Glycogen synthase kinase 3 - Promoter+5'UTR from <i>T. kok-saghyz</i>
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        </tr>
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    </td>
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        <tr>
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  </tr>
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            <td><a href='https://parts.igem.org/Part:BBa_K5088004'>BBa_K5088004</a></td>
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</table>
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            <td>Promoter + 5'UTR</td>
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            <td>P_betB</td>
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</body>
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            <td>Betaine-aldehyde dehydrogenase - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088005'>BBa_K5088005</a></td>
 +
            <td>Promoter + 5'UTR</td>
 +
            <td>P_pgm</td>
 +
            <td>Phosphoglucomutase - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088006'>BBa_K5088006</a></td>
 +
            <td>Promoter + 5'UTR</td>
 +
            <td>P_FKBP4_5</td>
 +
            <td>FK506-binding protein 4/5 - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088007'>BBa_K5088007</a></td>
 +
            <td>Promoter + 5'UTR</td>
 +
            <td>P_CLTC</td>
 +
            <td>Clathrin - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088008'>BBa_K5088008</a></td>
 +
            <td>Promoter + 5'UTR</td>
 +
            <td>P_RPL31</td>
 +
            <td>Large subunit ribosomal protein L31e - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088009'>BBa_K5088009</a></td>
 +
            <td>Promoter + 5'UTR</td>
 +
            <td>P_CUL1</td>
 +
            <td>Cullin - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088010'>BBa_K5088010</a></td>
 +
            <td>Promoter + 5'UTR</td>
 +
            <td>P_VPS4</td>
 +
            <td>Vacuolar protein-sorting-associated protein 4 - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088011'>BBa_K5088011</a></td>
 +
            <td>Promoter + 5'UTR</td>
 +
            <td>P_EIF2S3</td>
 +
            <td>Translation initiation factor 2 subunit 3 - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088012'>BBa_K5088012</a></td>
 +
            <td>Promoter + 5'UTR</td>
 +
            <td>P_Tubulin</td>
 +
            <td>Tubulin - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088013'>BBa_K5088013</a></td>
 +
            <td>Promoter + 5'UTR</td>
 +
            <td>P_EIF5A</td>
 +
            <td>Translation initiation factor 5A - Promoter+5'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088050'>BBa_K5088050</a></td>
 +
            <td>Inducible Promoter + 5'UTR</td>
 +
            <td>P_HSP12.6</td>
 +
            <td>HSP12.6 - Heat inducible promoter+5'UTR from <i>T. koksaghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088051'>BBa_K5088051</a></td>
 +
            <td>Inducible Promoter + 5'UTR</td>
 +
            <td>P_HSP23.5</td>
 +
            <td>HSP23.5 - Heat inducible promoter+5'UTR from <i>T. koksaghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088102'>BBa_K5088102</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_PTI1</td>
 +
            <td>Protein tyrosine kinase - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088103'>BBa_K5088103</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_RPL28</td>
 +
            <td>Large subunit ribosomal protein L28e - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088104'>BBa_K5088104</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_EPS15</td>
 +
            <td>Epidermal growth factor receptor substrate 15 - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088105'>BBa_K5088105</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_GSK3B</td>
 +
            <td>Glycogen synthase kinase 3 - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088106'>BBa_K5088106</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_MGRN1</td>
 +
            <td>E3 ubiquitin-protein ligase - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088107'>BBa_K5088107</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_RPL35A</td>
 +
            <td>Large subunit ribosomal protein L35Ae - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088108'>BBa_K5088108</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_betB</td>
 +
            <td>Betaine-aldehyde dehydrogenase - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088109'>BBa_K5088109</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_pgm</td>
 +
            <td>Phosphoglucomutase - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088110'>BBa_K5088110</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_ATP-synt</td>
 +
            <td>ATPase subunit gamma - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088111'>BBa_K5088111</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_EIF3B</td>
 +
            <td>Translation initiation factor 3 subunit B - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088112'>BBa_K5088112</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_RPL31</td>
 +
            <td>Large subunit ribosomal protein L31e - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088113'>BBa_K5088113</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_TM9SF2_4</td>
 +
            <td>Transmembrane 9 superfamily member 2/4 - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088114'>BBa_K5088114</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_CUL1</td>
 +
            <td>Cullin - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088115'>BBa_K5088115</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_PSMB6</td>
 +
            <td>20S proteasome subunit beta 1 - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088116'>BBa_K5088116</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_RPSA</td>
 +
            <td>Small subunit ribosomal protein SAe - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088117'>BBa_K5088117</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_VPS4</td>
 +
            <td>Vacuolar protein-sorting-associated protein 4 - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
        <tr>
 +
            <td><a href='https://parts.igem.org/Part:BBa_K5088118'>BBa_K5088118</a></td>
 +
            <td>3'UTR</td>
 +
            <td>T_EIF2S3</td>
 +
            <td>Translation initiation factor 2 subunit 3 - 3'UTR from <i>T. kok-saghyz</i></td>
 +
        </tr>
 +
      </table>
 +
  </body>
 
</html>
 
</html>
  

Revision as of 11:48, 11 August 2024


Protein tyrosine kinase - Promoter+5'UTR from T. kok-saghyz


Background

Motivation

Use Cases

Lack of Endogenous Regulatory Parts

Political Context

Plant Synthetic Biology

Gene Structure in Plants

Cis-Regulatory Elements

Design and Characterization

Identification & Design Strategy

Genetic Context of the Part

Figure X: Lorem ipsum dolor sit amen

Measurement

Dual Fluorescence Reporter Assay

Choice of Reporter Genes

Transient Transformation

Leaf Infiltration

Protoplast

Measurement Setup

Results

The Dandelion Toolbox

Overview

Part Identifier Part Type Nickname Part Description
BBa_K5088000 Promoter + 5'UTR P_PTI1 Protein tyrosine kinase - Promoter+5'UTR from T. kok-saghyz
BBa_K5088001 Promoter + 5'UTR P_RPL28 Large subunit ribosomal protein L28e - Promoter+5'UTR from T. kok-saghyz
BBa_K5088002 Promoter + 5'UTR P_GSK3B Glycogen synthase kinase 3 - Promoter+5'UTR from T. kok-saghyz
BBa_K5088003 Promoter + 5'UTR P_MGRN1 E3 ubiquitin-protein ligase - Promoter+5'UTR from T. kok-saghyz
BBa_K5088004 Promoter + 5'UTR P_betB Betaine-aldehyde dehydrogenase - Promoter+5'UTR from T. kok-saghyz
BBa_K5088005 Promoter + 5'UTR P_pgm Phosphoglucomutase - Promoter+5'UTR from T. kok-saghyz
BBa_K5088006 Promoter + 5'UTR P_FKBP4_5 FK506-binding protein 4/5 - Promoter+5'UTR from T. kok-saghyz
BBa_K5088007 Promoter + 5'UTR P_CLTC Clathrin - Promoter+5'UTR from T. kok-saghyz
BBa_K5088008 Promoter + 5'UTR P_RPL31 Large subunit ribosomal protein L31e - Promoter+5'UTR from T. kok-saghyz
BBa_K5088009 Promoter + 5'UTR P_CUL1 Cullin - Promoter+5'UTR from T. kok-saghyz
BBa_K5088010 Promoter + 5'UTR P_VPS4 Vacuolar protein-sorting-associated protein 4 - Promoter+5'UTR from T. kok-saghyz
BBa_K5088011 Promoter + 5'UTR P_EIF2S3 Translation initiation factor 2 subunit 3 - Promoter+5'UTR from T. kok-saghyz
BBa_K5088012 Promoter + 5'UTR P_Tubulin Tubulin - Promoter+5'UTR from T. kok-saghyz
BBa_K5088013 Promoter + 5'UTR P_EIF5A Translation initiation factor 5A - Promoter+5'UTR from T. kok-saghyz
BBa_K5088050 Inducible Promoter + 5'UTR P_HSP12.6 HSP12.6 - Heat inducible promoter+5'UTR from T. koksaghyz
BBa_K5088051 Inducible Promoter + 5'UTR P_HSP23.5 HSP23.5 - Heat inducible promoter+5'UTR from T. koksaghyz
BBa_K5088102 3'UTR T_PTI1 Protein tyrosine kinase - 3'UTR from T. kok-saghyz
BBa_K5088103 3'UTR T_RPL28 Large subunit ribosomal protein L28e - 3'UTR from T. kok-saghyz
BBa_K5088104 3'UTR T_EPS15 Epidermal growth factor receptor substrate 15 - 3'UTR from T. kok-saghyz
BBa_K5088105 3'UTR T_GSK3B Glycogen synthase kinase 3 - 3'UTR from T. kok-saghyz
BBa_K5088106 3'UTR T_MGRN1 E3 ubiquitin-protein ligase - 3'UTR from T. kok-saghyz
BBa_K5088107 3'UTR T_RPL35A Large subunit ribosomal protein L35Ae - 3'UTR from T. kok-saghyz
BBa_K5088108 3'UTR T_betB Betaine-aldehyde dehydrogenase - 3'UTR from T. kok-saghyz
BBa_K5088109 3'UTR T_pgm Phosphoglucomutase - 3'UTR from T. kok-saghyz
BBa_K5088110 3'UTR T_ATP-synt ATPase subunit gamma - 3'UTR from T. kok-saghyz
BBa_K5088111 3'UTR T_EIF3B Translation initiation factor 3 subunit B - 3'UTR from T. kok-saghyz
BBa_K5088112 3'UTR T_RPL31 Large subunit ribosomal protein L31e - 3'UTR from T. kok-saghyz
BBa_K5088113 3'UTR T_TM9SF2_4 Transmembrane 9 superfamily member 2/4 - 3'UTR from T. kok-saghyz
BBa_K5088114 3'UTR T_CUL1 Cullin - 3'UTR from T. kok-saghyz
BBa_K5088115 3'UTR T_PSMB6 20S proteasome subunit beta 1 - 3'UTR from T. kok-saghyz
BBa_K5088116 3'UTR T_RPSA Small subunit ribosomal protein SAe - 3'UTR from T. kok-saghyz
BBa_K5088117 3'UTR T_VPS4 Vacuolar protein-sorting-associated protein 4 - 3'UTR from T. kok-saghyz
BBa_K5088118 3'UTR T_EIF2S3 Translation initiation factor 2 subunit 3 - 3'UTR from T. kok-saghyz

Dandelion Handbook

References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1262
    Illegal BglII site found at 409
    Illegal XhoI site found at 436
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
    Illegal AgeI site found at 1761
  • 1000
    COMPATIBLE WITH RFC[1000]