Difference between revisions of "Part:BBa K5088000"

m
m
Line 13: Line 13:
  
 
==Background==
 
==Background==
−
<h3>Motivation</h3>
+
===Motivation===
−
<h4>Use Cases</h4>
+
====Use Cases====
−
<h4>Lack of Endogenous Regulatory Parts</h4>
+
====Lack of Endogenous Regulatory Parts====
−
<h4>Political Context</h4>
+
====Political Context====
  
−
<h3>Plant Synthetic Biology</h3>
+
===Plant Synthetic Biology===
−
<h4>Gene Structure in Plants</h4>
+
====Gene Structure in Plants====
−
<h4>Cis-Regulatory Elements</h4>
+
====Cis-Regulatory Elements====
  
 
==Design and Characterization==
 
==Design and Characterization==
−
<h3>Identification & Design Strategy</h3>
+
===Identification & Design Strategy===
  
 
==Genetic Context of the Part==
 
==Genetic Context of the Part==
Line 30: Line 30:
 
==Measurement==
 
==Measurement==
 
===Dual Fluorescence Reporter Assay===
 
===Dual Fluorescence Reporter Assay===
−
<h5>Choice of Reporter Genes</h5>
+
====Choice of Reporter Genes====
−
<h4>Transient Transformation</h4>
+
===Transient Transformation===
−
<h5>Leaf Infiltration</h5>
+
====Leaf Infiltration====
−
<h5>Protoplast</h5>
+
====Protoplast====
−
<h4>Measurement Setup</h4>
+
===Measurement Setup===
  
  
Line 41: Line 41:
  
 
==The Dandelion Toolbox==
 
==The Dandelion Toolbox==
−
<h3>Overview</h3>
+
===Overview===
−
<h3>Dandelion Handbook</h3>
+
===Dandelion Handbook===
  
 
==References==
 
==References==

Revision as of 16:01, 7 August 2024


Protein tyrosine kinase - Promoter+5'UTR from T. kok-saghyz


Background

Motivation

Use Cases

Lack of Endogenous Regulatory Parts

Political Context

Plant Synthetic Biology

Gene Structure in Plants

Cis-Regulatory Elements

Design and Characterization

Identification & Design Strategy

Genetic Context of the Part

Measurement

Dual Fluorescence Reporter Assay

Choice of Reporter Genes

Transient Transformation

Leaf Infiltration

Protoplast

Measurement Setup

Results

The Dandelion Toolbox

Overview

Dandelion Handbook

References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1262
    Illegal BglII site found at 409
    Illegal XhoI site found at 436
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
    Illegal AgeI site found at 1761
  • 1000
    COMPATIBLE WITH RFC[1000]