Difference between revisions of "Part:BBa K5088000"

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Line 16: Line 16:
 
<h4>Use Cases</h4>
 
<h4>Use Cases</h4>
 
<h4>Lack of Endogenous Regulatory Parts</h4>
 
<h4>Lack of Endogenous Regulatory Parts</h4>
−
<h4>Political context</h4>
+
<h4>Political Context</h4>
  
 
<h3>Plant Synthetic Biology</h3>
 
<h3>Plant Synthetic Biology</h3>
Line 25: Line 25:
 
<h3>Identification & Design Strategy</h3>
 
<h3>Identification & Design Strategy</h3>
  
−
<h3>Measurement</h3>
+
==Genetic Context of the Part==
−
<h4>Dual Fluorescence Reporter Assay</h4>
+
 
 +
 
 +
==Measurement==
 +
===Dual Fluorescence Reporter Assay===
 
<h5>Choice of Reporter Genes</h5>
 
<h5>Choice of Reporter Genes</h5>
 
<h4>Transient Transformation</h4>
 
<h4>Transient Transformation</h4>
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==The Dandelion Toolbox==
 
==The Dandelion Toolbox==
 
<h3>Overview</h3>
 
<h3>Overview</h3>
 +
<h3>Dandelion Handbook</h3>
  
 
==References==
 
==References==

Revision as of 15:20, 7 August 2024


Protein tyrosine kinase - Promoter+5'UTR from T. kok-saghyz


Background

Motivation

Use Cases

Lack of Endogenous Regulatory Parts

Political Context

Plant Synthetic Biology

Gene Structure in Plants

Cis-Regulatory Elements

Design and Characterization

Identification & Design Strategy

Genetic Context of the Part

Measurement

Dual Fluorescence Reporter Assay

Choice of Reporter Genes

Transient Transformation

Leaf Infiltration
Protoplast

Measurement Setup


Results

The Dandelion Toolbox

Overview

Dandelion Handbook

References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1262
    Illegal BglII site found at 409
    Illegal XhoI site found at 436
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
    Illegal AgeI site found at 1761
  • 1000
    COMPATIBLE WITH RFC[1000]