Difference between revisions of "Part:BBa K5088000"

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<h3>Motivation</h3>
 
<h3>Motivation</h3>
 
<h4>Use Cases</h4>
 
<h4>Use Cases</h4>
 +
<h4>Lack of Endogenous Regulatory Parts</h4>
 +
<h4>Political context</h4>
 +
 +
<h3>Plant Synthetic Biology</h3>
 +
<h4>Gene Structure in Plants</h4>
 +
<h4>Cis-Regulatory Elements</h4>
  
 
==Design and Characterization==
 
==Design and Characterization==
 +
<h3>Identification & Design Strategy</h3>
 +
 +
<h3>Measurement</h3>
 +
<h4>Dual Fluorescence Reporter Assay</h4>
 +
<h5>Choice of Reporter Genes</h5>
 +
<h4>Transient Transformation</h4>
 +
<h5>Leaf Infiltration</h5>
 +
<h5>Protoplast</h5>
 +
<h4>Measurement Setup</h4>
 +
  
 
==Results==
 
==Results==
 +
  
 
==The Dandelion Toolbox==
 
==The Dandelion Toolbox==
 +
<h3>Overview</h3>
  
 
==References==
 
==References==

Revision as of 14:14, 7 August 2024


Protein tyrosine kinase - Promoter+5'UTR from T. kok-saghyz


Background

Motivation

Use Cases

Lack of Endogenous Regulatory Parts

Political context

Plant Synthetic Biology

Gene Structure in Plants

Cis-Regulatory Elements

Design and Characterization

Identification & Design Strategy

Measurement

Dual Fluorescence Reporter Assay

Choice of Reporter Genes

Transient Transformation

Leaf Infiltration
Protoplast

Measurement Setup


Results

The Dandelion Toolbox

Overview

References

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1262
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1262
    Illegal BglII site found at 409
    Illegal XhoI site found at 436
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1262
    Illegal XbaI site found at 184
    Illegal AgeI site found at 1761
  • 1000
    COMPATIBLE WITH RFC[1000]