Difference between revisions of "Part:BBa K5143006"
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− | The fwYellow gene was synthesized and its nucleotide sequence optimized for synthesis and expression in <i>Saccharomyces cerevisiae</i>. | + | The fwYellow gene was synthesized and its nucleotide sequence optimized for synthesis and expression in <i>Saccharomyces cerevisiae</i>. We used the fwYellow fused to a yeast secretion signal, the alpha-factor (<a href="https://parts.igem.org/Part:BBa_K5143009">BBa_K5143009</a>) in 5' and to a cellulose binding domain (<a href="https://parts.igem.org/Part:BBa_K5143007">BBa_K5143007</a>) in 3'. See the composite part here : <a href="https://parts.igem.org/Part:BBa_K5143023">BBa_K5143023</a> |
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Latest revision as of 13:56, 1 August 2024
fwYellow, chromoprotein optimized for expression in Saccharomyces cerevisiae
Description
fwYellow is a chromoprotein that was synthetized to monitor biological processes, like fluorescent proteins, but without the need to use devices to detect the protein. Chromoproteins absorb visible light so that they are visible in the ambient light unlike fluorescent proteins. fwYellow was first synthetized by the iGEM team Uppsala in 2013 (BBa_K1033910). The excitation wavelength is 520nm and the emission wavelength is 540nm. Here, the fwYellow chromoprotein was transformed into Saccharomyces cerevisiae cocultured with the cellulose-producing bacteria Komagataeibacter rhaeticus in order to make the cellulose yellow. The coloured cellulose, that should also be sticky thanks to a bioglue (BBa_K5143022) would then trap insects that are specifically attracted to yellow.
![fwYellow](https://static.igem.wiki/teams/5143/bba-k5143006-fwyellow-gene.png)
![yellow cellulose](https://static.igem.wiki/teams/5143/bba-k5143006-yellow-cellulose.png)
Construction
The fwYellow gene was synthesized and its nucleotide sequence optimized for synthesis and expression in Saccharomyces cerevisiae. We used the fwYellow fused to a yeast secretion signal, the alpha-factor (BBa_K5143009) in 5' and to a cellulose binding domain (BBa_K5143007) in 3'. See the composite part here : BBa_K5143023
References
1. iGEM13_Uppsala
2. Liljeruhm, J. et al. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering 12, 8 (2018).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 427
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 427
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 427
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 427
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 427
- 1000COMPATIBLE WITH RFC[1000]