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	<h1>Description</h1>		<h1>Description</h1>
	<p>		<p>
−	ECRIRE DES TRUCS ICI POUR LA DESCRIPTION	+	fwYellow is a chromoprotein that was synthetized to monitor biological processes, like fluorescent proteins, but without the need to use devices to detect the protein. Chromoproteins absorb visible light so that they are visible in the ambient light unlike fluorescent proteins. fwYellow was first synthetized by the iGEM team Uppsala in 2013 (Part:BBa_K1033910). The excitation wavelength is 520nm and the emission wavelength is 540nm.
		+	Here, the fwYellow chromoprotein was transformed into Saccharomyces cerevisiae cocultured with the cellulose-producing bacteria Komagataeibacter rhaeticus in order to make the cellulose yellow. The coloured cellulose, that should also be sticky thanks to a bioglue (Part:BBa_K5143022) would then trap insects that are specifically attracted to yellow.
	</p>		</p>
	<img src="" width="400" alt="NOM PHOTO">		<img src="" width="400" alt="NOM PHOTO">
	<h1>Construction</h1>		<h1>Construction</h1>
	<p>		<p>
−	ECRIRE DES TRUCS ICI POUR LA CONSTRUCTION	+	The fwYellow gene was synthesized and its nucleotide sequence optimized for synthesis and expression in Saccharomyces cerevisiae.
	</p>		</p>
	<h1>References</h1>		<h1>References</h1>
	<p>		<p>
−	METTRE LES REF ICI	+	1. iGEM13_Uppsala
		+	2. Liljeruhm, J. et al. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering 12, 8 (2018).
		+
		+
	</p>		</p>
	</body>		</body>

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Revision as of 09:35, 1 August 2024

fwYellow, chromoprotein optimized for expression in Saccharomyces cerevisiae

Description

fwYellow is a chromoprotein that was synthetized to monitor biological processes, like fluorescent proteins, but without the need to use devices to detect the protein. Chromoproteins absorb visible light so that they are visible in the ambient light unlike fluorescent proteins. fwYellow was first synthetized by the iGEM team Uppsala in 2013 (Part:BBa_K1033910). The excitation wavelength is 520nm and the emission wavelength is 540nm. Here, the fwYellow chromoprotein was transformed into Saccharomyces cerevisiae cocultured with the cellulose-producing bacteria Komagataeibacter rhaeticus in order to make the cellulose yellow. The coloured cellulose, that should also be sticky thanks to a bioglue (Part:BBa_K5143022) would then trap insects that are specifically attracted to yellow.

Construction

The fwYellow gene was synthesized and its nucleotide sequence optimized for synthesis and expression in Saccharomyces cerevisiae.

References

1. iGEM13_Uppsala 2. Liljeruhm, J. et al. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of Biological Engineering 12, 8 (2018).

Sequence and Features