Difference between revisions of "Part:BBa K5143014"
(9 intermediate revisions by 2 users not shown) | |||
Line 3: | Line 3: | ||
<partinfo>BBa_K5143014 short</partinfo> | <partinfo>BBa_K5143014 short</partinfo> | ||
− | + | <html lang="en"> | |
+ | <head> | ||
+ | <meta charset="UTF-8"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | <title>Protein Description</title> | ||
+ | <style> | ||
+ | .image-container { | ||
+ | text-align: center; | ||
+ | margin: 20px auto; | ||
+ | border: 1px solid #000; | ||
+ | padding: 10px; | ||
+ | width: fit-content; | ||
+ | } | ||
+ | .image-container img { | ||
+ | width: 600px; /* Augmentez la taille de l'image ici */ | ||
+ | } | ||
+ | .image-caption { | ||
+ | text-align: center; | ||
+ | font-style: italic; | ||
+ | margin-top: 10px; | ||
+ | } | ||
+ | </style> | ||
+ | </head> | ||
+ | <body> | ||
+ | <h1>Description</h1> | ||
+ | <p> | ||
+ | mRuby2 is a red fluorescent protein that was engineered to monitor biochemical processes in living cells using changes in Fluorescence Resonance Energy Transfer (FRET). Therefore, mRuby2 can be used as a reporter system. | ||
+ | Here, mRuby2 was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pADH1 (<a href="https://parts.igem.org/Part:BBa_J63005">BBa_J63005</a>). | ||
+ | The excitation wavelength is 559nm and the emission wavelength is 600nm. | ||
+ | </p> | ||
+ | <div class="image-container"> | ||
+ | <img src="https://static.igem.wiki/teams/5143/bba-k5143014-mruby2.png" alt="mRuby2"> | ||
+ | <div class="image-caption">mRuby2 Red Fluorescent Protein used as a transcriptional reporter.</div> | ||
+ | </div> | ||
+ | <h1>Construction</h1> | ||
+ | <p> | ||
+ | The mRuby2 used was synthesized based on a sequence from a yeast toolkit so the sequence is optimized for expression in <i>Saccharomyces cerevisiae</i>. In our genetic construction, the gene mRuby2 was synthesized in fusion with the secretion signal AGA2 (<a href="https://parts.igem.org/Part:BBa_K5143020">BBa_K5143020</a>) in 5' and a 6XHis tag in 3'. See the composite part here: <a href="https://parts.igem.org/Part:BBa_K5143021">BBa_K5143021</a>. | ||
+ | </p> | ||
+ | <h1>References</h1> | ||
+ | <p> | ||
+ | 1. Lam, A. et al. Improving FRET dynamic range with bright green and red fluorescent proteins. Nat Methods 9, 1005–1012 (2012).<br> | ||
+ | 2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015). | ||
+ | </p> | ||
+ | </body> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | |||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 20:11, 31 July 2024
mRuby2 : reporter Red Fluorescent Protein
Description
mRuby2 is a red fluorescent protein that was engineered to monitor biochemical processes in living cells using changes in Fluorescence Resonance Energy Transfer (FRET). Therefore, mRuby2 can be used as a reporter system. Here, mRuby2 was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pADH1 (BBa_J63005). The excitation wavelength is 559nm and the emission wavelength is 600nm.
Construction
The mRuby2 used was synthesized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene mRuby2 was synthesized in fusion with the secretion signal AGA2 (BBa_K5143020) in 5' and a 6XHis tag in 3'. See the composite part here: BBa_K5143021.
References
1. Lam, A. et al. Improving FRET dynamic range with bright green and red fluorescent proteins. Nat Methods 9, 1005–1012 (2012).
2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]