Difference between revisions of "Part:BBa K5143015"
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<title>Protein Description</title> | <title>Protein Description</title> | ||
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<h1>Description</h1> | <h1>Description</h1> | ||
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| − | + | Venus is a Yellow Fluorescent Protein that was developed to overcome limitations to the use of Green Fluorescent Protein produced by Aequorea victoria. Therefore, Venus can be used as a reporter system. Here, Venus was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pGAP (Part:BBa_K4081994). The excitation wavelength is 516nm and the emission wavelength is 530nm. | |
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| + | <img src="https://static.igem.wiki/teams/5143/bba-k5143015-venus.png" width="800" alt="Venus"> <!-- Taille de l'image augmentée --> | ||
| + | <div class="image-caption">Venus Yellow Fluorescent Protein used as a transcriptional reporter.</div> | ||
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<h1>Construction</h1> | <h1>Construction</h1> | ||
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| − | + | The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (Part:BBa_K5143009) in 5'. See the composite part here: | |
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<h1>References</h1> | <h1>References</h1> | ||
<p> | <p> | ||
| − | + | 1. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87–90 (2002).<br> | |
| + | 2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015). | ||
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Latest revision as of 19:44, 31 July 2024
Venus : reporter Yellow Fluorescent Protein
Description
Venus is a Yellow Fluorescent Protein that was developed to overcome limitations to the use of Green Fluorescent Protein produced by Aequorea victoria. Therefore, Venus can be used as a reporter system. Here, Venus was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pGAP (Part:BBa_K4081994). The excitation wavelength is 516nm and the emission wavelength is 530nm.
Construction
The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (Part:BBa_K5143009) in 5'. See the composite part here:
References
1. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87–90 (2002).
2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]