Difference between revisions of "Part:BBa K187001"
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| − | pBA is one of two universal plasmids for the BioBytes standardized in vitro assembly method. The other universal plasmid, pAB, is entered as BBa_K187000. | + | pBA is one of two universal plasmids for the BioBytes standardized in vitro assembly method. The other universal plasmid, pAB, is entered as BBa_K187000. To prepare parts inserted in pBA for assembly, primers K187367 and K187368 should be used. |
The BioBytes system is based on a bead platform where constructs with long sticky ends are annealed together sequentially. This system is designed to enable the single day assembly of plasmids composed of up to about half a dozen premade parts. Complementary to existing BioBrick standards for DNA assembly, we expect this system to be useful for rapid generation of large constructs wherever an interpart spacing of 37 bp can be tolerated. | The BioBytes system is based on a bead platform where constructs with long sticky ends are annealed together sequentially. This system is designed to enable the single day assembly of plasmids composed of up to about half a dozen premade parts. Complementary to existing BioBrick standards for DNA assembly, we expect this system to be useful for rapid generation of large constructs wherever an interpart spacing of 37 bp can be tolerated. | ||
Using the plasmids pAB and pBA, as well as universal primer sequences for the PCR extraction of bricks from the plasmids, anyone will be able to use the BioBytes system for their own rapid assembly. The design ensures that the existing catalog of BioBrick parts is compatible with the system, and can be easily cloned into the universal plasmids for brick generation. We believe that this new system is ready for both use and formalization, and we have prepared a BBF RFC detailing the use of BioBytes. | Using the plasmids pAB and pBA, as well as universal primer sequences for the PCR extraction of bricks from the plasmids, anyone will be able to use the BioBytes system for their own rapid assembly. The design ensures that the existing catalog of BioBrick parts is compatible with the system, and can be easily cloned into the universal plasmids for brick generation. We believe that this new system is ready for both use and formalization, and we have prepared a BBF RFC detailing the use of BioBytes. | ||
Revision as of 04:19, 21 October 2009
pBA is one of two universal plasmids for the BioBytes standardized in vitro assembly method. The other universal plasmid, pAB, is entered as BBa_K187000. To prepare parts inserted in pBA for assembly, primers K187367 and K187368 should be used.
The BioBytes system is based on a bead platform where constructs with long sticky ends are annealed together sequentially. This system is designed to enable the single day assembly of plasmids composed of up to about half a dozen premade parts. Complementary to existing BioBrick standards for DNA assembly, we expect this system to be useful for rapid generation of large constructs wherever an interpart spacing of 37 bp can be tolerated.
Using the plasmids pAB and pBA, as well as universal primer sequences for the PCR extraction of bricks from the plasmids, anyone will be able to use the BioBytes system for their own rapid assembly. The design ensures that the existing catalog of BioBrick parts is compatible with the system, and can be easily cloned into the universal plasmids for brick generation. We believe that this new system is ready for both use and formalization, and we have prepared a BBF RFC detailing the use of BioBytes.