Difference between revisions of "Part:BBa K4765136"
 
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This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to ''E .coli'' BL21 DE3 and test its performance.
 
This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to ''E .coli'' BL21 DE3 and test its performance.
 
===Characterization===
 
===Characterization===
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====Agarose gel electrophoresis====
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{|
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| <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/ssb-tdp-xrcc1-dsup.png" alt="contributed by Fudan iGEM 2023"></html>
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|-
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| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
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From right lane(4) to left lane(1) indicate the successful construction of ''H. ex'' mtSSB, ''H. ex'' mtSSB + SAHS 33020, ''H. ex'' mtSSB + SAHS 33020 + XRCC1, and ''H. ex'' mtSSB + SAHS 33020 + XRCC1 + Dsup. '''
  
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|}
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4765136 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4765136 SequenceAndFeatures</partinfo>
  

Latest revision as of 16:00, 12 October 2023


ribozyme connected: FEN1 + H. ex mtSSB + SAHS 33020 + XRCC1 + Dsup

contributed by Fudan iGEM 2023

Usage and Biology

This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to E .coli BL21 DE3 and test its performance.

Characterization

Agarose gel electrophoresis

contributed by Fudan iGEM 2023
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

From right lane(4) to left lane(1) indicate the successful construction of H. ex mtSSB, H. ex mtSSB + SAHS 33020, H. ex mtSSB + SAHS 33020 + XRCC1, and H. ex mtSSB + SAHS 33020 + XRCC1 + Dsup.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3304
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 617
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1195
    Illegal NgoMIV site found at 1513
    Illegal AgeI site found at 301
    Illegal AgeI site found at 5095
    Illegal AgeI site found at 5166
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2146
    Illegal BsaI.rc site found at 1781
    Illegal BsaI.rc site found at 1862
    Illegal BsaI.rc site found at 4354
    Illegal BsaI.rc site found at 5092
    Illegal SapI site found at 1675
    Illegal SapI.rc site found at 5821