Difference between revisions of "Part:BBa K4765134"

 
 
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<partinfo>BBa_K4765134 short</partinfo>
 
<partinfo>BBa_K4765134 short</partinfo>
  
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ribozyme connected: Dsup + FEN1 + H. ex mtSSB + SAHS 33020 + XRCC1
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html>
  
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===Usage and Biology===
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This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to ''E .coli'' BL21 DE3 and test its performance.
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===Characterization===
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====Agarose gel electrophoresis====
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{|
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| <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/fen1-ssb-tdp-xrcc1.png" alt="contributed by Fudan iGEM 2023"></html>
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|-
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| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
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From right lane(3) to left lane(1) indicate the successful construction of Fen 1 + ''H. ex'' mtSSB, Fen 1 + ''H. ex'' mtSSB + SAHS 33020, and Fen 1 + ''H. ex'' mtSSB + SAHS 33020 + XRCC1. '''
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|}
 
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<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4765134 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4765134 SequenceAndFeatures</partinfo>
  

Latest revision as of 15:59, 12 October 2023


ribozyme connected: Dsup + FEN1 + H. ex mtSSB + SAHS 33020 + XRCC1

contributed by Fudan iGEM 2023

Usage and Biology

This part is the combination of all the anti-desiccation and anti-UV proteins, we've transformed it to E .coli BL21 DE3 and test its performance.

Characterization

Agarose gel electrophoresis

contributed by Fudan iGEM 2023
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

From right lane(3) to left lane(1) indicate the successful construction of Fen 1 + H. ex mtSSB, Fen 1 + H. ex mtSSB + SAHS 33020, and Fen 1 + H. ex mtSSB + SAHS 33020 + XRCC1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4573
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1886
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2464
    Illegal NgoMIV site found at 2782
    Illegal AgeI site found at 230
    Illegal AgeI site found at 301
    Illegal AgeI site found at 1570
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3415
    Illegal BsaI.rc site found at 227
    Illegal BsaI.rc site found at 3050
    Illegal BsaI.rc site found at 3131
    Illegal BsaI.rc site found at 5623
    Illegal SapI site found at 2944
    Illegal SapI.rc site found at 956