Difference between revisions of "Part:BBa K4765127"
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===Introduction===
 
===Introduction===
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Similar to [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126(ribozyme connected: ''H. ex'' mtSSB + AnAFP + SAHS 33020)], we changed the order of ''H. ex'' mtSSB, SAHS 33020 and AnAFP.
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Similar to [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126], we changed the order of ''H. ex'' mtSSB, SAHS 33020 and AnAFP.
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===Characterization===
 
===Characterization===
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Get details in [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126(ribozyme connected: ''H. ex'' mtSSB + AnAFP + SAHS 33020)].
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Get details in [https://parts.igem.org/Part:BBa_K4765126 BBa_K4765126].
 
====Agarose gel electrophoresis====
 
====Agarose gel electrophoresis====
 
{|
 
{|
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| <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/ssb-anafp-tdp.png" alt="contributed by Fudan iGEM 2023"></html>
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| <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/ssb-anafp-tdp.png" alt="contributed by Fudan iGEM 2023"></html>
 
|-
 
|-
 
| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.  
 
| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.  
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From left lane(1) to right lane(3) indicate the successful construction of H. ex mtSSB, H. ex mtSSB + AnAFP, and H. ex mtSSB + AnAFP + SAHS 33020. '''
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From left lane(1) to right lane(3) indicate the successful construction of ''H. ex'' mtSSB, ''H. ex'' mtSSB + AnAFP, and ''H. ex'' mtSSB + AnAFP + SAHS 33020. '''
  
 
|}
 
|}
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4765127 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4765127 SequenceAndFeatures</partinfo>
  

Latest revision as of 15:56, 12 October 2023


ribozyme connected: H. ex mtSSB + AnAFP + SAHS 33020 contributed by Fudan iGEM 2023

Introduction

Similar to BBa_K4765126, we changed the order of H. ex mtSSB, SAHS 33020 and AnAFP.

Characterization

Get details in BBa_K4765126.

Agarose gel electrophoresis

contributed by Fudan iGEM 2023
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

From left lane(1) to right lane(3) indicate the successful construction of H. ex mtSSB, H. ex mtSSB + AnAFP, and H. ex mtSSB + AnAFP + SAHS 33020.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1573
    Illegal BsaI.rc site found at 515
    Illegal BsaI.rc site found at 596
    Illegal SapI site found at 409
    Illegal SapI.rc site found at 1214