Difference between revisions of "Part:BBa K4765115"
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===Introduction=== | ===Introduction=== | ||
| − | This composite part is composed of FEN1 and ribozyme-assisted polycistronic co-expression system：pRAP. FEN1 plays a pivotal role in DNA maintenance, addressing 5' overhanging | + | This composite part is composed of FEN1 and ribozyme-assisted polycistronic co-expression system：pRAP. FEN1 plays a pivotal role in DNA maintenance, addressing 5' overhanging flaps formed when DNA strands can't bind correctly. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We constructed | + | We constructed FEN1 into pRAP to endow ''E. coli'' with anti-UV capability. |
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| − | + | ===Sequence and Features=== | |
<partinfo>BBa_K4765115 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4765115 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K4765115 parameters</partinfo> | <partinfo>BBa_K4765115 parameters</partinfo> | ||
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Latest revision as of 15:53, 12 October 2023
Twister P1 + T7_RBS + FEN1 + stem-loop
Introduction
This composite part is composed of FEN1 and ribozyme-assisted polycistronic co-expression system：pRAP. FEN1 plays a pivotal role in DNA maintenance, addressing 5' overhanging flaps formed when DNA strands can't bind correctly.
Usage and Biology
We constructed FEN1 into pRAP to endow E. coli with anti-UV capability.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 617
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1195
Illegal AgeI site found at 301 - 1000COMPATIBLE WITH RFC[1000]