Difference between revisions of "Part:BBa K4765115"
m |
|||
| (5 intermediate revisions by 3 users not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K4765115 short</partinfo> | <partinfo>BBa_K4765115 short</partinfo> | ||
| − | ribozyme | + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> |
| + | __TOC__ | ||
| + | |||
| + | ===Introduction=== | ||
| + | This composite part is composed of FEN1 and ribozyme-assisted polycistronic co-expression system：pRAP. FEN1 plays a pivotal role in DNA maintenance, addressing 5' overhanging flaps formed when DNA strands can't bind correctly. | ||
| + | |||
| + | ===Usage and Biology=== | ||
| + | We constructed FEN1 into pRAP to endow ''E. coli'' with anti-UV capability. | ||
| + | |||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
| Line 9: | Line 18: | ||
<!-- --> | <!-- --> | ||
| − | + | ===Sequence and Features=== | |
<partinfo>BBa_K4765115 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4765115 SequenceAndFeatures</partinfo> | ||
Latest revision as of 15:53, 12 October 2023
Twister P1 + T7_RBS + FEN1 + stem-loop
Introduction
This composite part is composed of FEN1 and ribozyme-assisted polycistronic co-expression system：pRAP. FEN1 plays a pivotal role in DNA maintenance, addressing 5' overhanging flaps formed when DNA strands can't bind correctly.
Usage and Biology
We constructed FEN1 into pRAP to endow E. coli with anti-UV capability.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 617
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1195
Illegal AgeI site found at 301 - 1000COMPATIBLE WITH RFC[1000]