Difference between revisions of "Part:BBa K4765113"

 
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<partinfo>BBa_K4765113 short</partinfo>
 
<partinfo>BBa_K4765113 short</partinfo>
  
ribozyme+T7_RBS+H. ex mtSSB+stem-loop
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html>
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__TOC__
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===Introduction===
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This composite part is composed of ''Hypsibius exemplaris'' mitochondrial single-stranded DNA binding protein (''H. ex'' mtSSB) and improved ribozyme-assisted polycistronic co-expression system:pRAP. ''H. ex'' mtSSB can coat ssDNA to limit damage during desiccation and UV radiation.
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===Usage and Biology===
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We constructed SAHS 33020 into pRAP to endow ''E. coli'' with desiccation-resistant and anti-UV capability. And this composite part can cooperate with [https://parts.igem.org/Part:BBa_K4765112 BBa_K4765112] to increase the survival rate of ''E.coli'' under desiccation.
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Get details in [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016].
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4765113 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4765113 SequenceAndFeatures</partinfo>
  

Latest revision as of 15:51, 12 October 2023


Twister P1 + T7_RBS + H. ex mtSSB + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of Hypsibius exemplaris mitochondrial single-stranded DNA binding protein (H. ex mtSSB) and improved ribozyme-assisted polycistronic co-expression system:pRAP. H. ex mtSSB can coat ssDNA to limit damage during desiccation and UV radiation.

Usage and Biology

We constructed SAHS 33020 into pRAP to endow E. coli with desiccation-resistant and anti-UV capability. And this composite part can cooperate with BBa_K4765112 to increase the survival rate of E.coli under desiccation.

Get details in BBa_K4765016.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 515
    Illegal BsaI.rc site found at 596
    Illegal SapI site found at 409