Difference between revisions of "Part:BBa K4765114"
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Latest revision as of 15:51, 12 October 2023
Twister P1 + T7_RBS + Rv Dsup + stem-loop
Introduction
This composite part is composed of Rv Dsup and ribozyme-assisted polycistronic co-expression system：pRAP. Dsup is a nucleosome-binding protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species Ramazzottius Varieornatus .
Usage and Biology
We constructed Rv Dsup into pRAP to endow E. coli with anti-UV capability.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 230
Illegal AgeI site found at 301 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 227
Illegal SapI.rc site found at 956