Difference between revisions of "Part:BBa K4765114"
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<partinfo>BBa_K4765114 short</partinfo> | <partinfo>BBa_K4765114 short</partinfo> | ||
| − | ribozyme | + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> |
| + | __TOC__ | ||
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| + | ===Introduction=== | ||
| + | This composite part is composed of Rv Dsup and ribozyme-assisted polycistronic co-expression system：pRAP. Dsup is a nucleosome-binding protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species ''Ramazzottius Varieornatus'' . | ||
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| + | ===Usage and Biology=== | ||
| + | We constructed Rv Dsup into pRAP to endow ''E. coli'' with anti-UV capability. | ||
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| − | + | ===Sequence and Features=== | |
<partinfo>BBa_K4765114 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4765114 SequenceAndFeatures</partinfo> | ||
Latest revision as of 15:51, 12 October 2023
Twister P1 + T7_RBS + Rv Dsup + stem-loop
Introduction
This composite part is composed of Rv Dsup and ribozyme-assisted polycistronic co-expression system：pRAP. Dsup is a nucleosome-binding protein that helps protect DNA from radiation damage. It was discovered in the Tardigrade Species Ramazzottius Varieornatus .
Usage and Biology
We constructed Rv Dsup into pRAP to endow E. coli with anti-UV capability.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 230
Illegal AgeI site found at 301 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 227
Illegal SapI.rc site found at 956