Difference between revisions of "Part:BBa K4765112"
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===Introduction===
 
===Introduction===
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This composite part is composed of Secretory-abundant heat soluble protein 33020 (SAHS 33020) and ribozyme-assisted polycistronic co-expression system：pRAP. SAHS 33020 is a heat-soluble protein in tardigrades that helps them survive tough conditions by forming a protective glass matrix during desiccation. It was tested by iGEM17_TUDelft in [https://parts.igem.org/Part:BBa_K2306003 Secretory-abundant heat soluble protein 33020 (SAHS 33020)].
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This composite part is composed of Secretory-abundant heat soluble protein 33020 (SAHS 33020) and improved ribozyme-assisted polycistronic co-expression system：pRAP. SAHS 33020 is a heat-soluble protein in tardigrades that helps them survive tough conditions by forming a protective glass matrix during desiccation. It was tested by iGEM17_TUDelft in [https://parts.igem.org/Part:BBa_K2306003 BBa_K2306003].
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===Usage and Biology===
 
===Usage and Biology===
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We constructed SAHS 33020 into pRAP to endow ‘’E. coli’’ with desiccation-resistant capability. And this composite part can cooperate with [https://parts.igem.org/Part:BBa_K4765113 ribozyme+T7_RBS+mtSSB+stem-loop] to increase the survival rate of ’’E.coli’’ under desiccation.
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We constructed SAHS 33020 into pRAP to endow ''E. coli'' with desiccation-resistant capability. And this composite part can cooperate with [https://parts.igem.org/Part:BBa_K4765113 BBa_K4765113] to increase the survival rate of ''E.coli'' under desiccation.
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===Characterization===
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Get details in [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016].
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4765112 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4765112 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K4765112 parameters</partinfo>
 
<partinfo>BBa_K4765112 parameters</partinfo>
 
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==Reference==
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===Reference===

Latest revision as of 15:49, 12 October 2023


Twister P1 + T7_RBS + SAHS 33020 + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of Secretory-abundant heat soluble protein 33020 (SAHS 33020) and improved ribozyme-assisted polycistronic co-expression system：pRAP. SAHS 33020 is a heat-soluble protein in tardigrades that helps them survive tough conditions by forming a protective glass matrix during desiccation. It was tested by iGEM17_TUDelft in BBa_K2306003.

Usage and Biology

We constructed SAHS 33020 into pRAP to endow E. coli with desiccation-resistant capability. And this composite part can cooperate with BBa_K4765113 to increase the survival rate of E.coli under desiccation.

Get details in BBa_K4765016.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 178


Reference