Difference between revisions of "Part:BBa K4765026"

Latest revision as of 15:29, 12 October 2023

OpenV3

contributed by Fudan iGEM 2023

Usage and Biology

Robust, flexible E. coli cloning vector. Designed to be compatible with multiple cloning strategies including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard. See details on stanford.freegenes.org.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2089

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4765026 short</partinfo>
 <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html>
 ===Usage and Biology===
-OpenV3 is a plasmid backbone with AmpR, we constructed all the 'test' parts into it.
+Robust, flexible ''E. coli'' cloning vector. Designed to be compatible with multiple cloning strategies including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard. See details on [https://stanford.freegenes.org/products/open-plasmids#genes stanford.freegenes.org].
 <!-- Add more about the biology of this part here
@@ Line 11: / Line 12: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+===Sequence and Features===
 <partinfo>BBa_K4765026 SequenceAndFeatures</partinfo>