Difference between revisions of "Part:BBa K4451009"

Latest revision as of 15:29, 12 October 2023

phiEco32 gp79

BBa_K4451009 encodes the gp79 protein from phiEco32 phage. This protein has been reported to interact with RNA polymerase and cause inhibition of sigma-70-dependent transcription (Savalia et al., 2008).

Description

A transcriptional inhibitor from phiEco32 phage, which is able to cause growth rate suppression in E. coli. The Gp79 gene encodes a ~10kDa protein that has been shown to bind to the core of the RNA polymerase σ70 holoenzyme, which prevents transcription bubble formation in many the housekeeping genes in E. coli and thus enacts global transcriptional downregulation. Whilst the exact molecular mechanism has yet to be elucidated, it is likely that Gp79 is utilised to switch the specificity of host RNA polymerase to that of phiEco32 late genes in the infection cycle (Savalia et al., 2008).

Figure 1: Mean log2(OD) against time for gp79, concentrations refer to the [IPTG] used to induce the pLac promoter regulating gp79.

Figure 1 shows that there's a decrease in growth of E. coli when they are transformed with a plasmid containing gp79. This shows that expression of gp79 in E. coli likely doesn't impact growth of E. coli.

Sequence and Features