Difference between revisions of "Part:BBa K4765007"
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===Introduction===
 
===Introduction===
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Nb3 is a variant of nanobody, a type of single-domain antibody<ref>Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041
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Nb3 is a variant of nanobody, a type of single-domain antibody<ref>Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. ''Cell, 174''(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041
 
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. It can interact with[https://parts.igem.org/Part:BBa_K4765006 Ag3] and the interaction between Ag-Nb can mediate specific adhesion of Escherichia coli.
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. It can interact with [https://parts.igem.org/Part:BBa_K4765006 BBa_K4765006(Ag3)] and the interaction between Ag-Nb can mediate specific binding between ''E.coli''.
  
 
===Usage and Biology===
 
===Usage and Biology===
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The combination of Nb3’s single-domain structure and the intimin surface display system allows the entirety of a highly specific, cell surface-bound adhesin to be encoded as a single fusion protein.
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The combination of Nb3's single-domain structure and the intimin surface display system allows the entirety of a highly specific, cell surface-bound adhesin to be encoded as a single fusion protein.
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===Characterization===
 
===Characterization===
  

Latest revision as of 15:15, 12 October 2023

Nb3, R4P43 from 10.1016/j.cell.2018.06.041

contributed by Fudan iGEM 2023

Introduction

Nb3 is a variant of nanobody, a type of single-domain antibody[1] . It can interact with BBa_K4765006(Ag3) and the interaction between Ag-Nb can mediate specific binding between E.coli.

Usage and Biology

The combination of Nb3's single-domain structure and the intimin surface display system allows the entirety of a highly specific, cell surface-bound adhesin to be encoded as a single fusion protein.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 166


Reference

  1. ↑ Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041