Difference between revisions of "Part:BBa K4719025"

 
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<partinfo>BBa_K4719025 short</partinfo>
 
<partinfo>BBa_K4719025 short</partinfo>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4719025 SequenceAndFeatures</partinfo>
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==Introduction==
 
==Introduction==
 
<b>Vilnius-Lithuania iGEM 2023</b> team's goal was to create <b> synthetic biology tools for <i>in vivo</i> alterations of <i>Komagataeibacter xylinus</i> bacterial cellulose polymer composition</b>. Firstly, we chose to produce a <b>cellulose-chitin copolymer</b> that would later be deacetylated, creating <b>bacterial cellulose-chitosan</b>. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of the bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design. As a second approach, we designed <b>indigo-dyed cellulose</b> that could be used as a green chemistry way to apply cellulose in the textile industry. Lastly, we have achieved a of <b>bacterial cellulose and polyhydroxybutyrate (PHB) composite</b>, which is synthesized by <i>K. xylinus</i>.
 
<b>Vilnius-Lithuania iGEM 2023</b> team's goal was to create <b> synthetic biology tools for <i>in vivo</i> alterations of <i>Komagataeibacter xylinus</i> bacterial cellulose polymer composition</b>. Firstly, we chose to produce a <b>cellulose-chitin copolymer</b> that would later be deacetylated, creating <b>bacterial cellulose-chitosan</b>. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of the bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design. As a second approach, we designed <b>indigo-dyed cellulose</b> that could be used as a green chemistry way to apply cellulose in the textile industry. Lastly, we have achieved a of <b>bacterial cellulose and polyhydroxybutyrate (PHB) composite</b>, which is synthesized by <i>K. xylinus</i>.
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<h2> Usage and Biology</h2>
 
<h2> Usage and Biology</h2>
 
<p>
 
<p>
Proteomic analysis of ''K. xylinus'' was performed and signal peptide sequence was identified.  
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Proteomic analysis of <i>K. xylinus</i> was performed and signal peptide sequence was identified.  
 
</p>
 
</p>
 
<figure>
 
<figure>
 
<div class = "center" >
 
<div class = "center" >
<center><img src = "https://static.igem.wiki/teams/4719/wiki/partai/output-sequence-plot.png" style = "width:300px;"></center>
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<center><img src = "https://static.igem.wiki/teams/4719/wiki/partai/output-sequence-plot.png" style = "width:600px;"></center>
 
</div>
 
</div>
 
<figcaption><center><b>Figure 1.</b>SignalIP 6.0 output </center></figcaption>
 
<figcaption><center><b>Figure 1.</b>SignalIP 6.0 output </center></figcaption>
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4719025 SequenceAndFeatures</partinfo>
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Latest revision as of 15:08, 12 October 2023

K. xylinus extracellular signal peptide sequence
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

Vilnius-Lithuania iGEM 2023 team's goal was to create synthetic biology tools for in vivo alterations of Komagataeibacter xylinus bacterial cellulose polymer composition. Firstly, we chose to produce a cellulose-chitin copolymer that would later be deacetylated, creating bacterial cellulose-chitosan. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of the bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design. As a second approach, we designed indigo-dyed cellulose that could be used as a green chemistry way to apply cellulose in the textile industry. Lastly, we have achieved a of bacterial cellulose and polyhydroxybutyrate (PHB) composite, which is synthesized by K. xylinus.


Usage and Biology

Proteomic analysis of K. xylinus was performed and signal peptide sequence was identified.

Figure 1.SignalIP 6.0 output