Difference between revisions of "Part:BBa K4719025"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K4719025 short</partinfo> | <partinfo>BBa_K4719025 short</partinfo> | ||
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K4719025 SequenceAndFeatures</partinfo> | ||
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==Introduction== | ==Introduction== | ||
<b>Vilnius-Lithuania iGEM 2023</b> team's goal was to create <b> synthetic biology tools for <i>in vivo</i> alterations of <i>Komagataeibacter xylinus</i> bacterial cellulose polymer composition</b>. Firstly, we chose to produce a <b>cellulose-chitin copolymer</b> that would later be deacetylated, creating <b>bacterial cellulose-chitosan</b>. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of the bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design. As a second approach, we designed <b>indigo-dyed cellulose</b> that could be used as a green chemistry way to apply cellulose in the textile industry. Lastly, we have achieved a of <b>bacterial cellulose and polyhydroxybutyrate (PHB) composite</b>, which is synthesized by <i>K. xylinus</i>. | <b>Vilnius-Lithuania iGEM 2023</b> team's goal was to create <b> synthetic biology tools for <i>in vivo</i> alterations of <i>Komagataeibacter xylinus</i> bacterial cellulose polymer composition</b>. Firstly, we chose to produce a <b>cellulose-chitin copolymer</b> that would later be deacetylated, creating <b>bacterial cellulose-chitosan</b>. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of the bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design. As a second approach, we designed <b>indigo-dyed cellulose</b> that could be used as a green chemistry way to apply cellulose in the textile industry. Lastly, we have achieved a of <b>bacterial cellulose and polyhydroxybutyrate (PHB) composite</b>, which is synthesized by <i>K. xylinus</i>. | ||
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<h2> Usage and Biology</h2> | <h2> Usage and Biology</h2> | ||
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| − | Proteomic analysis of | + | Proteomic analysis of <i>K. xylinus</i> was performed and signal peptide sequence was identified. |
</p> | </p> | ||
<figure> | <figure> | ||
<div class = "center" > | <div class = "center" > | ||
| − | <center><img src = "https://static.igem.wiki/teams/4719/wiki/partai/output-sequence-plot.png" style = "width: | + | <center><img src = "https://static.igem.wiki/teams/4719/wiki/partai/output-sequence-plot.png" style = "width:600px;"></center> |
</div> | </div> | ||
<figcaption><center><b>Figure 1.</b>SignalIP 6.0 output </center></figcaption> | <figcaption><center><b>Figure 1.</b>SignalIP 6.0 output </center></figcaption> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Latest revision as of 15:08, 12 October 2023
K. xylinus extracellular signal peptide sequence
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 75
- 1000COMPATIBLE WITH RFC[1000]
Introduction
Vilnius-Lithuania iGEM 2023 team's goal was to create synthetic biology tools for in vivo alterations of Komagataeibacter xylinus bacterial cellulose polymer composition. Firstly, we chose to produce a cellulose-chitin copolymer that would later be deacetylated, creating bacterial cellulose-chitosan. This polymer is an easily modifiable platform when compared to bacterial cellulose. The enhanced chemical reactivity of the bacterial cellulose-chitosan polymer allows for specific functionalizations in the biomedicine field, such as scaffold design. As a second approach, we designed indigo-dyed cellulose that could be used as a green chemistry way to apply cellulose in the textile industry. Lastly, we have achieved a of bacterial cellulose and polyhydroxybutyrate (PHB) composite, which is synthesized by K. xylinus.
Usage and Biology
Proteomic analysis of K. xylinus was performed and signal peptide sequence was identified.
