Difference between revisions of "Part:BBa K4759071"
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===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 15:06, 12 October 2023
petH-RBS2-petF(D67W)
Usage and Biology
The P450 enzymes are redox-dependent proteins, through which they source electrons from reducing cofactors to drive their activities. This part is coding for ferredoxin reductase PetH and ferredoxin PetF from the algae (Synechocystis PCC 6803) as the redox chaperones of OleP. After obtaining the best redox partners PetH/PetF, we performed alanine scanning on petF to speculate which sites had a greater impact on its electron transport capacity. Finally, we found that after mutations in seven of them, the electron transport effect would change greatly, so we mutated the amino acids of these sites into other 19 amino acids by modeling, and selected 23 of them to get better results.
Fig. 1: Fermentation of 23 mutants and control groups We conducted control tests with the positive control group, negative control group, and wild-type strains, and finally selected 9 mutants with the highest fluorescence intensity for subsequent catalytic verification by detecting their green fluorescence intensity.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1249
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1249
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1249
- 1000COMPATIBLE WITH RFC[1000]