Difference between revisions of "Part:BBa K4613006"

Latest revision as of 14:30, 12 October 2023

Ptra

TraR protein forms a complex with its signaling molecule. This complex binds to the P_tra promoter, increasing the rate of transcription. Compared with BBa_K553002, we have truncated the sequence to 76 bp, including the tra box II、-35 sequences、-10 sequences and an RBS.

Reference

White C E, Winans S C.Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription[J].Mol Microbiol,2005, 55 (5): 1473-86.
Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 14
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4613006 short</partinfo>
-TraR protein forms a complex with its signaling molecule. This complex binds to the <em>Ptra</em> promoter, increasing the rate of transcription. Compared with BBa_K553002, we have truncated the sequence to 76 bp, including the tra box II&#12289;-35 sequences&#12289;-10 sequences and an RBS.
+TraR protein forms a complex with its signaling molecule. This complex binds to the <em>P<sub>tra</sub></em> promoter, increasing the rate of transcription. Compared with BBa_K553002, we have truncated the sequence to 76 bp, including the tra box II&#12289;-35 sequences&#12289;-10 sequences and an RBS.
 ==== Reference ====
-#White C E, Winans S C.Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription[J].Mol Microbiol,2005, 55 (5): 1473-86.
+#White C E, Winans S C.Identification of amino acid residues of the <i>Agrobacterium tumefaciens</i> quorum-sensing regulator TraR that are critical for positive control of transcription[J].Mol Microbiol,2005, 55 (5): 1473-86.
-#Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.
+#Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of <i>Agrobacterium tumefaciens</i> conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.
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