Difference between revisions of "Part:BBa K4166013"
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RFP under the control of Rainbow trout estrogen receptor by way of TetR: This is our biosensor for xenoestrogens like DDT. In the presence of such molecules, rtER will prevent expression of the TetR gene, which in turn relieves repression of RFP. In this case, TetR is an inverter module allowing for a red color signal in response to the xenoestrogen | RFP under the control of Rainbow trout estrogen receptor by way of TetR: This is our biosensor for xenoestrogens like DDT. In the presence of such molecules, rtER will prevent expression of the TetR gene, which in turn relieves repression of RFP. In this case, TetR is an inverter module allowing for a red color signal in response to the xenoestrogen | ||
| − | + | == Characterization of DDT Biosensor - Alma 2023 == | |
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Revision as of 14:27, 12 October 2023
DDT biosensor (RtER-TetR-RFP)
RFP under the control of Rainbow trout estrogen receptor by way of TetR: This is our biosensor for xenoestrogens like DDT. In the presence of such molecules, rtER will prevent expression of the TetR gene, which in turn relieves repression of RFP. In this case, TetR is an inverter module allowing for a red color signal in response to the xenoestrogen
Characterization of DDT Biosensor - Alma 2023
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1922
Illegal NheI site found at 1945 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 908
Illegal BamHI site found at 570
Illegal XhoI site found at 464 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3305
Illegal AgeI site found at 3417 - 1000COMPATIBLE WITH RFC[1000]