Difference between revisions of "Part:BBa K4683018:Experience"
 
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Gel Experimentation
 
Gel Experimentation
 
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We performed RCA on these six miRNAs (miR-20b, miR-328,miR-146, miR-150, miR-122, miR-30c) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
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We designed the padlock and the miRNA sequence, but unfortunately, we were unable to characterize these parts due to lack of time.  
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<html><img src="https://static.igem.wiki/teams/4683/wiki/rca-optimization/screenshot-2023-10-11-at-4-49-47-pm.png"
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alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
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Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146, miR-150, miR-122, and miR-30c; visible bands can be seen near the top of the wells.
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===User Reviews===
 
===User Reviews===

Latest revision as of 14:24, 12 October 2023


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4683018

Lambert_GA 2023
Gel Experimentation
We designed the padlock and the miRNA sequence, but unfortunately, we were unable to characterize these parts due to lack of time.

User Reviews

UNIQ2274ff2db0c439ab-partinfo-00000000-QINU UNIQ2274ff2db0c439ab-partinfo-00000001-QINU