Difference between revisions of "Part:BBa K4844007"

 
(Replaced content with " __NOTOC__ <partinfo>BBa_K4844005 short</partinfo> <!-- Add more about the biology of this part here ===Usage and Biology=== <!-- --> <span class='h3bb'>Sequence and F...")
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K4844005 short</partinfo>
+
<partinfo>BBa_K4844007 short</partinfo>
  
−
Usage:
 
−
Plants usually have severe responses under the pressure of low phosphate. Some genes will be turned on to  allow plants to better adapt to this situation. Therefore, in order to build a low-cost, real-time soil phosphate sensor, we first found a low phosphate response promoter in Maize which was reported by Jianrong Bai in 2018. Based on Bai's result, a 1502bp length promoter P1502-ZmPHR1 (BBa_K4844002, a new part submitted by our team in 2023) shows the strongest promoting strength under low phosphate pressure. Therefore, we decided to choose it as our low phosphate detection part. The sensor vector will be transformed into plants later with our "TTTT" system. (More about TTTT can be found at https://2023.igem.wiki/sz-shd/plant )
 
  
−
When the plant was under low phosphate stress. The P1502-ZmPHR1 promoter will up-regulate its' expression, the signal then enters the amplifier system, which eventually yields a large quantity of super bright eyGFP_UV (BBa_K4844000, new basic part submitted by our team in 2023) protein that can be seen under UV light with the naked eye.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 13: Line 10:
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K4844005 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K4844007 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K4844005 parameters</partinfo>
+
<partinfo>BBa_K4844007 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 14:23, 12 October 2023


Low phosphate phytosensor with low noise amplifier gene circuit


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1331
    Illegal XbaI site found at 277
    Illegal XbaI site found at 398
    Illegal PstI site found at 3145
    Illegal PstI site found at 3732
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1331
    Illegal NheI site found at 749
    Illegal NheI site found at 3506
    Illegal NheI site found at 3848
    Illegal NheI site found at 6885
    Illegal PstI site found at 3145
    Illegal PstI site found at 3732
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1331
    Illegal BglII site found at 5547
    Illegal BglII site found at 8903
    Illegal BamHI site found at 5313
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1331
    Illegal XbaI site found at 277
    Illegal XbaI site found at 398
    Illegal PstI site found at 3145
    Illegal PstI site found at 3732
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1331
    Illegal XbaI site found at 277
    Illegal XbaI site found at 398
    Illegal PstI site found at 3145
    Illegal PstI site found at 3732
    Illegal NgoMIV site found at 3502
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3485
    Illegal SapI.rc site found at 3622