Difference between revisions of "Part:BBa K4907141"

 
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<partinfo>BBa_K4907141 short</partinfo>
 
<partinfo>BBa_K4907141 short</partinfo>
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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===Biology===
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pBAD/araC promoter consists of two structures, namely araC and araBAD promoter. We found that <partinfo>BBa_K206001</partinfo> and araBAD promoter part overlapped, so we tried to combine <partinfo>BBa_K206001</partinfo> with the unoverlapped part to build an araBAD-weak promoter.
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===Usage===
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We constructed composite part:BBa_K4907141.
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<center><html><img src="https://static.igem.wiki/teams/4907/wiki/parts/yxy/arabad-weak-b0034-ccdb-1.png" width="400px"></html></center>
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<center><b>Fig. 1: Gene Circuit of BBa_K4907141</b></center>
  
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===Characterization===
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<span class='h3bb'>Sequence and Features</span>
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====Colony PCR====
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In the construction of this circuit, colony PCR and gene sequencing were used to verify the correctness of the transformants. At a position around 916bp, a target band of approximately 1000bp was observed (Fig. 2).
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<center><html><img src="https://static.igem.wiki/teams/4907/wiki/parts/yxy/parts/parts/bba-k4907141.png" width="400px"></html></center>
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<center><b>Fig. 2 DNA gel electrophoresis of the colony PCR products of BBa_K4907141_pSB4k5</b></center>
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===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K4907141 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4907141 SequenceAndFeatures</partinfo>
  

Latest revision as of 14:12, 12 October 2023


araBAD-weak-B0034-ccdB

Biology

pBAD/araC promoter consists of two structures, namely araC and araBAD promoter. We found that BBa_K206001 and araBAD promoter part overlapped, so we tried to combine BBa_K206001 with the unoverlapped part to build an araBAD-weak promoter.

Usage

We constructed composite part:BBa_K4907141.

Fig. 1: Gene Circuit of BBa_K4907141

Characterization

Colony PCR

In the construction of this circuit, colony PCR and gene sequencing were used to verify the correctness of the transformants. At a position around 916bp, a target band of approximately 1000bp was observed (Fig. 2).

Fig. 2 DNA gel electrophoresis of the colony PCR products of BBa_K4907141_pSB4k5

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 299
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 546
    Illegal SapI site found at 56