Difference between revisions of "Part:BBa K4960012"
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<partinfo>BBa_K4960012 short</partinfo>
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<partinfo>BBa_K4960012 short</partinfo><br>
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Pvc12 is a part of pvc baseplate.
 
===Profile===
 
===Profile===
 
Name: pvc12<br>
 
Name: pvc12<br>
 
Base Pairs: 2853 bp<br>
 
Base Pairs: 2853 bp<br>
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Origin: Photorhabdus<br>
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Origin:<i> Photorhabdus</i><br>
 
Properties: Pvc12 is a part of pvc baseplate.<br>
 
Properties: Pvc12 is a part of pvc baseplate.<br>
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<!-- Add more about the biology of this part here
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<!-- Add more about the biology of this part here-->
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===Usage and Biology===
 
===Usage and Biology===
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)<br>
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]('''Figure1''')<br>
 
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Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
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'''Figure 1. Schematic of the PVCpnf locus.''' It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
 
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In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of Pvc11 and the domain III of Pvc12 from a neighboring heterodimer.<br>
 
In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of Pvc11 and the domain III of Pvc12 from a neighboring heterodimer.<br>

Latest revision as of 14:07, 12 October 2023


pvc12
Pvc12 is a part of pvc baseplate.

Profile

Name: pvc12
Base Pairs: 2853 bp
Origin: Photorhabdus
Properties: Pvc12 is a part of pvc baseplate.

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of Pvc11 and the domain III of Pvc12 from a neighboring heterodimer.
Two sides of hexameric rings are found opposite surface charges, PVC12 included.
The evolutionary relationship between Pvc12 and known wedge proteins is not apparent because of the low sequence conservation.
In the six helices of the Pvc11Pvc12 dimer, four of which in Pvc12 are equivalent to the ones seen in the gp6-gp7 dimer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 855
    Illegal AgeI site found at 639
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.