Difference between revisions of "Part:BBa K4960007"
(→‎Profile)
 
(3 intermediate revisions by one other user not shown)
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K4960007 short</partinfo>
+
<partinfo>BBa_K4960007 short</partinfo><br>
−
===Properties===
+
guide the synthesis of the PVC tube initiator
 +
===Profile===
 +
Name: pvc7<br>
 +
Base Pairs: 690 bp<br>
 +
Origin: <i>Photorhabdus</i><br>
 +
Properties: guide the synthesis of the PVC tube initiator<br>
  
−
guide the synthesis of the PVC tube initiator.
+
<!-- Add more about the biology of this part here-->
  
−
<!-- Add more about the biology of this part here-->
 
 
===Usage and Biology===
 
===Usage and Biology===
−
Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Fig.a,Fig.b)<br>
+
Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]('''Figure1''')<br>
 
<html>
 
<html>
  
 
<figure class="figure">
 
<figure class="figure">
−
<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc2-3-4-5-6-7.png" class="figure-img img-fluid rounded"  height="200px">
+
<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
  
 
</figure>
 
</figure>
  
 
</html>
 
</html>
−
a. PVC illustrations <br>
+
'''Figure 1. Schematic of the PVCpnf locus.''' It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
−
b.Payload illustrations<br>
+
<br>
−
The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc7 produces pvc7 hexameric rings to form the first two initiator rings in the baseplate end of the PVC tube with pvc5  through opposite surface charges and electrostatic attraction(Fig.c).In the meantime,the pvc7 layer (appear to be fusion protein) docks onto the b-barrel ring (pseudo-6-fold) of Pvc8 to help form a continuous central  
+
The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc7 produces pvc7 hexameric rings to form the first two initiator rings in the baseplate end of the PVC tube with pvc5  through opposite surface charges and electrostatic attraction.In the meantime,the pvc7 layer (appear to be fusion protein) docks onto the b-barrel ring (pseudo-6-fold) of Pvc8 to help form a continuous central spike extending from the inner tube.<br>
−
spike extending from the inner tube.Furthermore, pvc7 is encircled by pvc9 and acts as a sheath initiator collectively to prepare for the contraction in the delivery process.(Fig.d)
+
Furthermore, pvc7 is encircled by pvc9 and acts as a sheath initiator collectively to prepare for the contraction in the delivery process.
−
<html>
+
  
−
<figure class="figure">
 
−
<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc-1-5-7.png" class="figure-img img-fluid rounded"  height="200px">
 
−
 
−
</figure>
 
−
 
−
</html>
 
−
c.the direction of pvc7<br>
 
−
d.The cross-sectional view of pvc
 
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===Sequence and Features===
 
<partinfo>BBa_K4960007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4960007 SequenceAndFeatures</partinfo>
  
Line 41: Line 36:
 
<!-- -->
 
<!-- -->
 
===References===
 
===References===
−
[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;<br>
+
[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
−
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;
+
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>

Latest revision as of 14:04, 12 October 2023


pvc7
guide the synthesis of the PVC tube initiator

Profile

Name: pvc7
Base Pairs: 690 bp
Origin: Photorhabdus
Properties: guide the synthesis of the PVC tube initiator


Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc7 produces pvc7 hexameric rings to form the first two initiator rings in the baseplate end of the PVC tube with pvc5 through opposite surface charges and electrostatic attraction.In the meantime,the pvc7 layer (appear to be fusion protein) docks onto the b-barrel ring (pseudo-6-fold) of Pvc8 to help form a continuous central spike extending from the inner tube.
Furthermore, pvc7 is encircled by pvc9 and acts as a sheath initiator collectively to prepare for the contraction in the delivery process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.