Difference between revisions of "Part:BBa K4683021:Experience"

 
 
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===Applications of BBa_K4683021===
 
===Applications of BBa_K4683021===
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<b> Lambert_GA 2023</b>
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Gel Experimentation
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We performed RCA on three miRNAs (miR-20b, miR-328,miR-146) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
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<html><img src="https://static.igem.wiki/teams/4683/wiki/human-practices/igem-estrogen-label.png"
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alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
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" width="500"></html>
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Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146a; visible bands can be seen near the top of the wells.
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===User Reviews===
 
===User Reviews===
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Latest revision as of 14:02, 12 October 2023


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Please enter how you used this part and how it worked out.

Applications of BBa_K4683021

Lambert_GA 2023
Gel Experimentation
We performed RCA on three miRNAs (miR-20b, miR-328,miR-146) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146a; visible bands can be seen near the top of the wells.


User Reviews

UNIQd309ac24cccdfb65-partinfo-00000001-QINU UNIQd309ac24cccdfb65-partinfo-00000002-QINU