Difference between revisions of "Part:BBa K4960001"

 
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<partinfo>BBa_K4960001 short</partinfo>
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<partinfo>BBa_K4960001 short</partinfo><br>
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guide the synthesis of the PVC tube
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===Profile===
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Name: pvc1<br>
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Base Pairs: 450bp<br>
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Origin: <i>Photorhabdus</i> <br>
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Properties: guide the synthesis of the PVC tube<br>
  
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]
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The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc1 produces pvc1 hexameric rings which are assembled to form main tube proteins through opposite surface charges and electrostatic attraction.As a whole,the inner surface of Pvc1 tube is remarkably negatively charged for loading its payloads.
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Furthermore,pvc1 interacts with the part of sheath comprises alternating Pvc2 and Pvc3 subunits ,which appears to be dictated by electrostatic interactions as well.This aims to prepare for the contraction in the delivery process.
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===Usage and Biology===
 
===Usage and Biology===
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]('''Figure1''')<br>
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
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'''Figure 1. Schematic of the PVCpnf locus.''' It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
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<br>
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The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc1 produces pvc1 hexameric rings which are assembled to form main tube proteins through opposite surface charges and electrostatic attraction.As a whole,the inner surface of Pvc1 tube is remarkably negatively charged for loading its payloads.<br>
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Furthermore,pvc1 interacts with the part of sheath comprises alternating Pvc2 and Pvc3 subunits ,which appears to be dictated by electrostatic interactions as well.This aims to prepare for the contraction in the delivery process.
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4960001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4960001 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K4960001 parameters</partinfo>
 
<partinfo>BBa_K4960001 parameters</partinfo>
 
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===References===
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>

Latest revision as of 14:01, 12 October 2023


pvc1
guide the synthesis of the PVC tube

Profile

Name: pvc1
Base Pairs: 450bp
Origin: Photorhabdus
Properties: guide the synthesis of the PVC tube


Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc1 produces pvc1 hexameric rings which are assembled to form main tube proteins through opposite surface charges and electrostatic attraction.As a whole,the inner surface of Pvc1 tube is remarkably negatively charged for loading its payloads.
Furthermore,pvc1 interacts with the part of sheath comprises alternating Pvc2 and Pvc3 subunits ,which appears to be dictated by electrostatic interactions as well.This aims to prepare for the contraction in the delivery process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.