Difference between revisions of "Part:BBa K4759204:Design"
| (One intermediate revision by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | Add a solubilization-promoting tag at the N-terminal of the P450 enzyme | |
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | company | + | The solubilization-promoting tag SUMO was purchased from company |
===References=== | ===References=== | ||
Latest revision as of 13:58, 12 October 2023
T7-RBS4-SUMO-linker-OleP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 295
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 295
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 295
Illegal BglII site found at 184 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 295
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 295
Illegal AgeI site found at 420
Illegal AgeI site found at 518 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Add a solubilization-promoting tag at the N-terminal of the P450 enzyme
Source
The solubilization-promoting tag SUMO was purchased from company