Difference between revisions of "Part:BBa K4683023:Experience"
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===Applications of BBa_K4683023=== | ===Applications of BBa_K4683023=== | ||
− | <b> Lambert_GA | + | <b> Lambert_GA 2023</b> |
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Gel Experimentation | Gel Experimentation |
Revision as of 13:03, 12 October 2023
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Applications of BBa_K4683023
Lambert_GA 2023
Gel Experimentation
We performed RCA on these six miRNAs (miR-20b, miR-328,miR-146, miR-150, miR-122, miR-30c) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146, miR-150, miR-122, and miR-30c; visible bands can be seen near the top of the wells.
User Reviews
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