Difference between revisions of "Part:BBa K4825018:Design"
 
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===Design Notes===
 
===Design Notes===
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"1. Different spacers derived from the article of synthetic promoters are placed between Inv AraI1 and AraO2 to ensure the original 193 bp interval present in natural E.coli is conserved. AraO2 is one bp upstream from TSS to explore different oppotunities.
 
"1. Different spacers derived from the article of synthetic promoters are placed between Inv AraI1 and AraO2 to ensure the original 193 bp interval present in natural E.coli is conserved. AraO2 is one bp upstream from TSS to explore different oppotunities.
 
2.Inversion of Ara operon with two AraI half sites reverses the original position of AraO. The inversion reduces the potential repressive effect due to dimeric AraC binding to AraI half sites in the Ara operon.
 
2.Inversion of Ara operon with two AraI half sites reverses the original position of AraO. The inversion reduces the potential repressive effect due to dimeric AraC binding to AraI half sites in the Ara operon.
 
3.T-rich spacers results in nucleosome-free regions to reduce the potential impacts from neighbouring DNA.
 
3.T-rich spacers results in nucleosome-free regions to reduce the potential impacts from neighbouring DNA.
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===References===
 
===References===
 +
 
"Robert Schleif. Regulation of the l-arabinose operon of Escherichia coli. Trends in Genetics, Volume 16, Issue 12, 2000, Pages 559-565, ISSN 0168-9525, https://doi.org/10.1016/S0168-9525(00)02153-3.
 
"Robert Schleif. Regulation of the l-arabinose operon of Escherichia coli. Trends in Genetics, Volume 16, Issue 12, 2000, Pages 559-565, ISSN 0168-9525, https://doi.org/10.1016/S0168-9525(00)02153-3.
  
 
Schleif R. A Career's Work, the l-Arabinose Operon: How It Functions and How We Learned It. EcoSal Plus. 2022 Dec 15;10(1):eESP00122021. doi: 10.1128/ecosalplus.ESP-0012-2021. Epub 2021 Aug 18. PMID: 36519894."
 
Schleif R. A Career's Work, the l-Arabinose Operon: How It Functions and How We Learned It. EcoSal Plus. 2022 Dec 15;10(1):eESP00122021. doi: 10.1128/ecosalplus.ESP-0012-2021. Epub 2021 Aug 18. PMID: 36519894."

Latest revision as of 13:03, 12 October 2023


pAra1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 46
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

"1. Different spacers derived from the article of synthetic promoters are placed between Inv AraI1 and AraO2 to ensure the original 193 bp interval present in natural E.coli is conserved. AraO2 is one bp upstream from TSS to explore different oppotunities. 2.Inversion of Ara operon with two AraI half sites reverses the original position of AraO. The inversion reduces the potential repressive effect due to dimeric AraC binding to AraI half sites in the Ara operon. 3.T-rich spacers results in nucleosome-free regions to reduce the potential impacts from neighbouring DNA. 

"


Source

Synthesized

References

"Robert Schleif. Regulation of the l-arabinose operon of Escherichia coli. Trends in Genetics, Volume 16, Issue 12, 2000, Pages 559-565, ISSN 0168-9525, https://doi.org/10.1016/S0168-9525(00)02153-3.

Schleif R. A Career's Work, the l-Arabinose Operon: How It Functions and How We Learned It. EcoSal Plus. 2022 Dec 15;10(1):eESP00122021. doi: 10.1128/ecosalplus.ESP-0012-2021. Epub 2021 Aug 18. PMID: 36519894."