Difference between revisions of "Part:BBa K4800019"

 
 
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<partinfo>BBa_K4800019 short</partinfo>
 
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EutM-SpyCatcher proteins can spontaneously assemble into axial protein fibres, with high binding efficiency between SpyTag and SpyCatcher. Allows scaffold proteins to join with enzyme elements to form a complex. Increases the stability of the enzyme element.
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We constructed EutM-SpyCatcher building blocks and attached itself covalently via isopeptide bond formation by the SpyTag-SpyCatcher system to the formed scaffolds，joining with enzyme elements to form a complex and improving the stability of the enzyme element.
  
 
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 12:53, 12 October 2023


EutM-SpyCatcher

We constructed EutM-SpyCatcher building blocks and attached itself covalently via isopeptide bond formation by the SpyTag-SpyCatcher system to the formed scaffolds，joining with enzyme elements to form a complex and improving the stability of the enzyme element.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 42
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]