Difference between revisions of "Part:BBa K283023"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K283023 short</partinfo>
 
<partinfo>BBa_K283023 short</partinfo>
  
−
This part contains a strong rbs and a TetR tagged with a LVA degradation tail. The TetR repressor can be used to repress ptet promoter and the TetR coding region do not contain any terminator
+
Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator before cheZ gene. We intend use this device to control the expression of cheZ and bactomotors' movement.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:14, 20 October 2009

plux-rbs-CI-terminator-plambda(R-O12)-rbs-cheZ-terminator

Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator before cheZ gene. We intend use this device to control the expression of cheZ and bactomotors' movement.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal NheI site found at 2472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
    Illegal BglII site found at 332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 307
    Illegal EcoRI site found at 1792
  • 1000
    COMPATIBLE WITH RFC[1000]