Difference between revisions of "Part:BBa K4645020"
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| − | <p>The circuit of the adhesion part is as follows (<b>Figure 1</b>).</p> | + | <p>The design circuit of the adhesion part is as follows (<b>Figure 1</b>).</p> |
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<img src=https://static.igem.wiki/teams/4645/wiki/project/design/sterilization-circuit.png width=100% alt=""> | <img src=https://static.igem.wiki/teams/4645/wiki/project/design/sterilization-circuit.png width=100% alt=""> | ||
Revision as of 12:34, 12 October 2023
The two-component system of S. mutans senses the CSP to regulate the expression of ClyR
CSP is a quorum sensing signaling molecule of S. mutans, and its concentration is positively correlated with the amount of S. mutans. ComD is a membrane-bound histidine kinase (HK) capable of autophosphorylation in response to sensing CSP. The response regulatory factor (RR) ComE then catalyzes the transfer of phosphorylated groups to aspartic residues in its receptor region. Phosphorylated ComE (P-ComE) is allosterically transformed into an active conformation that binds upstream to the CSP-sensitive promoter nlmC and activates transcription. Then expressing ClyR, a chimeric lysin, is composed of a catalytic domain from PlyC and a cell-wall binding domain from PlySs2. ClyR can recognize and lyse the cell wall of S. mutans and avoids the biofilm formed by S. mutans on the surface of our bacteria.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 627
Illegal EcoRI site found at 1985
Illegal XbaI site found at 3288
Illegal PstI site found at 1027 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 627
Illegal EcoRI site found at 1985
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 1027 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 627
Illegal EcoRI site found at 1985
Illegal XhoI site found at 3240 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 627
Illegal EcoRI site found at 1985
Illegal XbaI site found at 3288
Illegal PstI site found at 1027 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 627
Illegal EcoRI site found at 1985
Illegal XbaI site found at 3288
Illegal PstI site found at 1027
Illegal AgeI site found at 2608
Illegal AgeI site found at 2692
Illegal AgeI site found at 3007
Illegal AgeI site found at 3190 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1823
Illegal BsaI.rc site found at 2262
Gene Design in Circuits
The design circuit of the adhesion part is as follows (Figure 1).
