Difference between revisions of "Part:BBa K4800012"

Latest revision as of 12:25, 12 October 2023

ackA-pta sgRNA

SgRNA, which is small guide RNA, is an important component of the CRISPR knockdown system. The N20 sequence of the ackA-pta sgRNA was derived from the ackA and pta genes and could be localised to the acka-pta gene in the Escherichia coli NT1003 genome to assist the Cas9 protein in silencing the gene.

Document

Build:

Fig1. ackA-pta sgRNA

We designed the N20 sequence of ackA-pta by using the CHOPCHOP website (http://chopchop.cbu.uib.no), synthesized them on primers and ligated the N20 into the pTarget-sgRNA plasmid by means of PCR.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Unknown
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4800012 short</partinfo>
-SgRNA, which is small guide RNA, is an important component of the CRISPR knockdown system. The N20 sequence of the ackA-pta SgRNA was derived from the ackA and pta genes and could be localised to the acka-pta gene in the <i>Escherichia coli</i> NT1003 genome to assist the Cas9 protein in silencing the gene.
+SgRNA, which is small guide RNA, is an important component of the CRISPR knockdown system. The N20 sequence of the ackA-pta sgRNA was derived from the ackA and pta genes and could be localised to the acka-pta gene in the <i>Escherichia coli</i> NT1003 genome to assist the Cas9 protein in silencing the gene.
 <!-- Add more about the biology of this part here
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      <p style="font-size: 150%; font-weight: bold;">Build:
-     <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/acka-pta-sgrna.png" width="80%" height="80%"></p>
+     <p><center><img src="https://static.igem.wiki/teams/4800/wiki/parts/acka-pta-sgrna.png" width="50%" height="50%"></center></p>
      <div align="center">
          <strong>Fig1. ackA-pta sgRNA</strong>