Difference between revisions of "Part:BBa K4765021"
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In the 2022 iGEM competition, we successfully constructed pRAP using the hammerhead ribozyme to modify the metabolism of ''E. coli'' for β-carotene production. This year, we have discovered that the mono-cistron mRNA generated by the ribozyme self-cleavage is unstable. Therefore, we have added stem-loop 1 at the 3' end of the corresponding CDS to prevent the degradation of mono-cistron mRNA. | In the 2022 iGEM competition, we successfully constructed pRAP using the hammerhead ribozyme to modify the metabolism of ''E. coli'' for β-carotene production. This year, we have discovered that the mono-cistron mRNA generated by the ribozyme self-cleavage is unstable. Therefore, we have added stem-loop 1 at the 3' end of the corresponding CDS to prevent the degradation of mono-cistron mRNA. | ||
| − | + | ===Characterization=== | |
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Latest revision as of 12:23, 12 October 2023
stem-loop 1, from 10.1021/acssynbio.2c00416
Introduction
The stem-loop is a piece of RNA that forms the stem loop structure, which is located at the 3' end of the mono-cistron cut by ribozyme, preventing the degradation of mono-cistron mRNA.[1].
Usage and Biology
In the 2022 iGEM competition, we successfully constructed pRAP using the hammerhead ribozyme to modify the metabolism of E. coli for β-carotene production. This year, we have discovered that the mono-cistron mRNA generated by the ribozyme self-cleavage is unstable. Therefore, we have added stem-loop 1 at the 3' end of the corresponding CDS to prevent the degradation of mono-cistron mRNA.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416